乌司他丁对急性脑出血模型兔sTM、vWF及脑组织病理的影响  

Effect of Ulinastatin on sTM and vWF and Tissue Pathology of the Brain of Rabbits with Acute Cerebral Hemorrhage

作　　者：佟丽妍[1] 史秀丽 徐焕凤 冯为民[1] 

机构地区：[1]杭州市中医院神经内科,杭州310007 [2]河北省玉田县计生中心医院优生办公室,唐山064100 [3]河北省石家庄长江心理医院中医科,石家庄050000

出　　处：《浙江中西医结合杂志》2016年第10期898-901,I0002,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药卫生科技计划研究项目(No.2014ZB091);天普科学研究基金资助项目(No.01200919)

摘　　要：目的通过对急性脑出血模型兔动脉血可溶性血栓调节蛋白(sTM)、血管性假性血友病因子(vWF)以及脑组织标本病理变化的观察,探讨乌司他丁(UTI)在急性脑出血中的脑保护作用。方法将造模后的30只兔随机分为脑出血组、依达拉奉组、乌司他丁组,每组10只。应用ELISA法测定各组兔急性脑出血后动脉血sTM、vWF的含量,以及兔脑出血病灶周围脑组织病理变化。结果与脑出血组[sTM 24h:(6.06±0.19)ng/m L,72h:(6.42±0.48)ng/m L;vWF 24h:(10.02±0.71)ng/m L,72h:(11.39±0.63)ng/m L]比较,依达拉奉组sTM含量[24h:(4.61±0.11)ng/m L,72h:(4.51±0.10)ng/m L]均有下降,差异有统计学意义(P<0.05);依达拉奉组vWF含量在72h([9.64±0.56)ng/m L]时下降,差异有统计学意义(P<0.05);乌司他丁组sTM、vWF含量[24h:sTM(4.82±0.77)ng/m L,vWF(9.09±0.24)ng/m L;72h:sTM(4.79±0.75)ng/m L,vWF(8.48±0.73)ng/m L]均下降,差异有统计学意义(P<0.05)。依达拉奉组与乌司他丁组比较,24h、72h sTM含量差异均无统计学意义(P>0.05),24h及72h vWF含量比较差异均有统计学意义(P<0.05)。病理结果提示,乌司他丁组脑出血病灶周围脑细胞的线粒体嵴断裂,胞浆内空泡增多,细胞肿胀方面均比依达拉奉组延缓。结论乌司他丁可以降低急性脑出血模型兔血清sTM及vWF含量,以及出血灶周围脑细胞线粒体及细胞核的损害,具有一定的脑保护作用。Objective To observe arterial blood soluble thrombomodulin（sTM）, von Willebrand factor（vWF） and pathological changes of brain tissue specimens in rabbits with acute cerebral hemorrhage after ulinastatin（UTI） intervention, in an attempt to investigate the preventive effect of UTI on the brain. Methods Thirty rabbits successfully modeled were randomly divided into 3 groups： intracerebral hemorrhage（ICH） model group（M group）, edaravone treatment group（Y group）, and UTI treatment group（U group）, with 10 rabbits in each group. ELISA method was used to determine the content of vWF and sTM in the arterial blood and the pathological changes of brain tissues around acute cerebra[ hemorrhage in the rabbits were observed. Results Compared with those of M group [sTM （24h： 6.06±0.19 ng/mL, 72h： 6.42±0.48 ng/mL）; vWF（24h： 10.02 ±0.71 ng/mL, 72h： 11.39±0.63 ng/mL）], Y group had significantly lower content of sTM at the 24h（4.61±0.11 ng/mL） and 72h（4.51±0.10 ng/ mL, with P〈0.05 and lower content of vWF at 72h （4.51±0.10 ng/mL）, with P〈0.05; U group had both reduced contents of sTM and vWF at 24h（sTM： 4.82±0.77 ng/mL, vWF： 9.09±0.24 ng/mL） and 72h（sTM： 4.79±0.75 ng/ mL, vWF： 8.48±0.73 ng/mL）, all with signigicant differences （all P〈0.05）. No significant difference in sTM at both time points was found between Y group and U group （P〉0.05）, while significant differences in vWF was noted at both time points between the two groups （P〈0.05）. Pathological results showed that the cells around the lesion of ICH in both U and Y groups appeared mitochondria erista broken and cytoplasmic vacuoles increased and cell swelling, but that those in U group were obviously lighter than those in Y group. Conclusion UTI can reduce serum content of sTM and vWF in acute ICH model rabbit, delay the damage of mitochondria and nucleus of the ceils surrounding focal hemorrhage, which indicates that UTI has a certain brain protective effect.

关 键 词：兔 急性脑出血 乌司他丁 可溶性血栓调节蛋白 血管性假性血友病因子 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]