转化生长因子β1和溶血磷脂酸增强整合素αvβ6在肝细胞性肝癌中的表达  

Expression of transforming growth factor β1 and lysophosphatidic acid increasing the activation of integrin αvβ6 in hepatic cancer

作　　者：韩慧[1] 许锐锐[1] 陈耿臻[1] 吴成亮[1] 郑凯欣[1] 黄泽鸿[1] 

机构地区：[1]汕头大学医学院第二附属医院微创医学中心,515041

出　　处：《中华普通外科学文献（电子版）》2016年第5期340-345,共6页Chinese Archives of General Surgery(Electronic Edition)

基　　金：广东省医学科研基金项目(B2013275)

摘　　要：目的探讨转化生长因子β1(TGF-β1)和溶血磷脂酸(LPA)是否能诱导整合素αvβ6在肝细胞性肝癌中的过度表达。方法收集23例肝细胞癌患者的组织标本,采用免疫组织化学方法检测整合素αvβ6蛋白的表达水平。培养人肝癌细胞Hep-3B,以正常肝细胞(HL-7702)为对照,采用逆转录PCR(RT-PCR)法检测整合素αvβ6表达,再分别用TGF-β1和(或)LPA刺激Hep-3B细胞,Western Blotting法检测整合素αvβ6蛋白的表达水平,实时荧光定量PCR(Real-time PCR)法检测Hep-3B中整合素αvβ6的m RNA表达水平。结果 (1)免疫组织化学检测显示整合素αvβ6在肝癌标本中呈高表达,而在正常肝脏组织中并不表达。(2)逆转录PCR检测Hep-3B细胞表达整合素αvβ6,在正常肝细胞中未发现表达。(3)Western Blotting、实时定量PCR结果显示TGF-β1、LPA和TGF-β1+LPA均可诱导Hep-3B细胞过度表达整合素αvβ6,差异有统计学意义(P<0.05)。结论整合素αvβ6在肝癌组织及Hep-3B细胞中呈高表达。TGF-β1和LPA可诱导整合素αvβ6在Hep-3B细胞中过度表达,这将有望为肝癌的诊断和治疗提供新的潜在治疗靶点。Objective To investigate whether transforming growth gactor-beta1（TGF-β1） andlysophospholipids acid（LPA） can induce the overexpression of integrin αvβ6（Itg αvβ6） in human hepaticcancer Hep-3B. Methods Tissue samples were obtained from twenty three patients with hepatocellularcarcinoma, and the expression level of Itg αvβ6 was detected by immunohistochemistry. The expression ofItg αvβ6 in Hep-3B cells and normal liver cells HL-7702 was treated with revised transcription PCR（RT-PCR）. Hep-3B cells were treated with either TGF-β1, LPA, or TGF-β1＋LPA, then Itg αvβ6 expressionwas examined by real-time PCR and Western blotting. Results αvβ6 overexpression was present in thehepatocellular carcinoma, while absent in normal liver tissue. αvβ6 m RNA was detected in Hep-3B cellsbut not detectable in HL-7702 cells. TGF-β1, LPA and TGF-β1＋LPA induced Itg avβ6 overexpression atprotein levels and m RNA levels, with a significant difference（P〈0.05）. Conclusions αvβ6 overexpressionis present in both the hepatocellular carcinoma and Hep-3B cells. TGF-β1 and LPA induce the Itg αvβ6overexpression in Hep-3B cells, which will be expected to provide a new potential therapeutic target forhepatocellular carcinoma.

关 键 词：癌 肝细胞 整合素ΑVΒ6 转化生长因子Β1 溶血磷脂素类 

分 类 号：R735.7[医药卫生—肿瘤]