急性髓细胞白血病免疫分型及骨髓、血常规综合研究  

Immunophenotyping and bone marrow hematology analysis in acute myelocytic leukemia

作　　者：郭斌[1] 谢宁[1] 刘文[1] 李英[1] 李君安[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000

出　　处：《国际检验医学杂志》2016年第19期2710-2712,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨骨髓细胞形态学、外周血液学和免疫分型对急性髓细胞白血病诊疗的临床意义。方法显微镜检查骨髓细胞形态学,全自动血细胞分析仪检测外周血相关参数,流式细胞计数仪分析白血病细胞免疫标志物,并对所有数据进行统计分析。结果外周血细胞约85%的患者提示异常或幼稚细胞的存在,不同类型白血病其白细胞总数变化差异明显（M1-2、M5以增高为主,M3以减低为主）;多数患者是贫血和血小板减少,但程度不同;白细胞分类中,仪器无法分类鉴别急性粒细胞和急性单核细胞,淋巴系统免疫标志物CD7、CD10和CD2可交叉表达于髓系白血病,对治疗和预后有临床作用。结论外周血相关参数的检测对白血病的早期筛查、鉴别诊断、预后判断均有临床意义,而免疫标志物检测是骨髓细胞形态学诊断和分型的补充和支持,且异常敏感标志物的差异表达跟预后密切相关,可显著提高白血病的诊治率。Objective To investigate the clinical significance in diagnosis and prognostic judgments of acute myelocytic leukemia with combined detection of morphology,peripheral blood and immune typing of bone marrow cells.Methods Microscopic examination of bone marrow cell morphology,automatic blood cell analyzer detection of peripheral blood parameter and flow cytometry analysis of immune markers of leukemia cells,all these datas were analyzed statistically.Results Peripheral blood cell analysis showed that instruments may indicate the presence of abnormal or naive cells in about 85% patients,different kinds of leukemia had significant difference in total leukocyte count（majority higher in M1-2,M5;majority lower in M3）;while anemia and thrombocytopenia were observed in most patients,but the degree was different;M1-2and M5 can not be identified by classification of leukocyte by automatic blood cell analyzer,CD7,CD10 and CD2can cross express in myeloid leukemia,which have a prompt effect with treatment and prognosis.Conclusion Detection of peripheral blood parameters has an important role in early screening,differential diagnosis and prognosis judgment of leukemia,immunological markers detection is the powerful supplement and support for leukemia diagnosis and typing,sensitive markers have close contact with prognosis,which significantly improves the effect of the diagnosis and treatment of leukemia.

关 键 词：急性髓细胞白血病 骨髓细胞形态 外周血 免疫分型 

分 类 号：R733.71[医药卫生—肿瘤]