连香树精油对植物病原真菌抑菌作用及对人肝癌细胞株SMMC-7721细胞毒性研究  

Evaluation of Antibacterial and Cytotoxic Activity of Cercidiphyllum japonicum Oil Against Phytopathogenic Fungi and Humanhepatocellular Carcinoma Cells SMMC-7721

作　　者：汪超[1] 唐明[1] 魏琴[2] 马丹炜[1] 王亚男[1] 张红[1] 李群[1] 

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]宜宾学院香料植物资源开发与利用四川省高校重点实验室,四川宜宾644000

出　　处：《四川师范大学学报（自然科学版）》2016年第5期735-742,共8页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省高校重点实验室开放基金(2015XLY006)

摘　　要：首次以连香树精油作为天然抑菌剂及抑癌剂,检测连香树精油对6种植物病原真菌的抑菌作用及对人肝癌细胞株SMMC-7721的细胞毒性,旨在探究连香树精油在抑菌和抑癌方面的功能,为天然抗菌剂和抗癌药物的开发打下基础.采用改良琼脂稀释法测定连香树精油对6种植物病原真菌的抑制率、最低抑菌体积分数(MIC)和最小杀菌体积分数(MBC),并采用MTT法检测其对人体肝癌细胞SMMC-7721的细胞毒性.结果表明,连香树精油具有较好的抗菌性和抗癌性.在抗菌方面,对玉米大斑、黑曲霉以及根霉的抑菌效果较好,抑制率分别达到97.8%、99.7%、99.4%;对禾谷镰孢菌的抑菌效果较差(最高抑制率仅为29.1%).在MIC和MBC测定实验中,同样表明连香树精油对黑曲霉的抑菌效果较好(MIC为6.25μL/m L,MBC为50μL/m L).在抑癌方面,连香树精油对SMMC-7721肝癌细胞具有较好的抑制效果,当精油体积分数为8μL/m L时,对SMMC-7721肝癌细胞的抑制率可以达到67.9%,与阳性对照5-氟尿嘧啶(抑制率68.9%)没有显著差异,且对正常肝细胞L02的杀伤作用(抑制率18.7%)要远远低于5-氟尿嘧啶对其伤害(抑制率59%).连香树精油对植物病原真菌和SMMC-7721肝癌细胞具有良好的抑制作用,在农药及医药应用中有较好的应用潜力.This is the first study to use Cercidiphyllum japonicum oil as natural antimicrobial agents and anticancer drugs. To detect the antibacterial activity of Cercidiphyllum japonicum oil on the 6 kinds of phytopathogenic fungis as well as the cytotoxic function against human carcinoma cells,SMMC-7721,this study explores the efficacy of Cercidiphyllum japonicum oil on antibacterial and antitumor properties and lays the foundation for the development of natural antimicrobial agents and anticancer drugs. Using the modified agar diffusion method,the inhibitory rate,the minimal inhibitory concentration（ MIC） and the minimal bactericidal concentration（ MBC）of concentration（ MIC） against the 6 kinds of pathogenic fungi have been investigated. In order to detect the relevant cytotoxic effects on human carcinoma cells,SMMC-7721,the MTT method is used. The Cercidiphyllum japonicum oil has an incredible effect on the antimicrobial and anticancer properties. In terms of the antibacterial aspect of Setosphaeria,the Exserohilum turcicum,Aspergillus Niger and Rhizopus,had better inhibitory effects,with the inhibition rates reaching 97. 8%,99. 7% and 99. 4%,respectively. However,the Fusarium graminearum showed a poorer effect（ with the highest inhibition rate reaching only 29. 1%）. The result from the experiment of the 6 kinds of pathogenic fungi with the method of MIC and MBC also shows that Cercidiphyllum japonicum oil performs well when dealing with Aspergillus Niger（ MIC is 6. 25 μL/m L,MBC is 50 μL/m L）. The Cercidiphyllum japonicum oil also has good inhibition effects on human carcinoma cells,SMMC-7721. When the concentration is 8 μL/m L,the inhibition rates can reach up to67. 9%. No significant differences have been found when it was compared to positive control 5-fluorouraci（ inhibition rate 59%）. Cercidiphyllum japonicum oil has an excellent inhibitory effect on phytopathogenic fungi and human carcinoma cells,SMMC-7721,which indicates a high potential in the future,in the areas of pesticide and me

关 键 词：连香树精油 天然抗菌剂 天然抗癌剂 植物病原真菌 SMMC-7721肝癌细胞 

分 类 号：Q939.95[生物学—微生物学]