CRYAB在喉癌中的表达变化及临床意义  

Differentiated expression and clinical significance of CRYAB in LSCC

作　　者：许里[1] 徐新宇[1] 林振中[1] 

机构地区：[1]江苏省肿瘤医院病理科,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2016年第9期1095-1100,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81301951);江苏省六大人才高峰项目(2015-WSN-017);南京市科委科技发展项目(201402055)

摘　　要：目的:检测晶状体蛋白-αB(crystallin-alpha B,CRYAB)在人喉癌中的表达情况,同时研究CRYAB表达与喉癌重要临床指标之间的关系。方法:在10例新鲜喉癌及癌旁组织上进行一步法定量逆转录PCR(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)检测,在组织芯片上(80例喉癌标本及20例癌旁对照标本)进行免疫组化检测,使用Cox回归法及Kaplan-Meier生存分析法进行喉癌患者的预后评估。结果 :qPCR及免疫组化结果均显示喉癌组织中的CRYAB表达明显高于癌旁对照组织中CRYAB的表达(P均<0.05)。CRYAB的表达与喉癌患者的肿瘤分化程度(P=0.022)、TNM分期(P=0.013)、淋巴结转移(P=0.026)及生存情况(P=0.001)有重要关联。生存分析显示CRYAB表达(P=0.036)与喉癌患者预后关系密切,CRYAB高表达提示患者预后不良。结论 :CRYAB在喉癌组织中异常高表达,且与喉癌患者的数种恶性表型相关,可作为喉癌患者预后的独立危险因素。Objective： To examine the expression of crystallin-alpha B（CRYAB） in human laryngeal squamous cell carcinoma（LSCC） and investigate the relationship between its expression and clinical characteristics of LSCC. Methods： One-step quantitative reverse transcription-polymerase chain reaction（q RT-PCR） test in 10 fresh LSCCs as well as non-cancerous tissue samples and immunohistochemistry analysis by tissue microarrays（80 LSCC samples and 20 tumor-adjacent normal samples） were performed to characterize CRYAB expression in LSCC. Cox regression and Kaplan-Meier survival analyses were carried out to evaluate the prognosis of LSCC. Results： The results of q PCR test and immunohistochemistry analysis showed that the expression of CRYAB in LSCC was significantly higher than that in tumor-adjacent normal tissues（P = 0.0005 and P = 0.021, respectively）. Moreover, the expression level of CRYAB protein in LSCC was significantly related to tumor differentiation（P = 0.022）, TNM stage（P = 0.013）,lymph node metastasis（P = 0.026） and overall survival（P = 0.001）. COX multi-factor analysis showed that CRYAB expression（P =0.036） was recognized as an independent prognosis factors for LSCC. Conclusion： The data suggest that CRYAB expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.

关 键 词：喉癌 CRYAB qPCR 免疫组化 预后 

分 类 号：R739.65[医药卫生—肿瘤]