MiRNA-138-5p抑制人胰腺癌细胞增殖能力  

MiRNA-138-5p inhibits proliferation of pancreatic cancer cells

作　　者：王杰[1] 喻超[1] 周显飞 江建新[1] 

机构地区：[1]贵州医科大学附属医院肝胆外科,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2016年第28期3970-3977,共8页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81160311;81572429;81660483~~

摘　　要：目的探讨微小RNA(microRNA,miR)-138-5p对胰腺癌(pancreatic cancer,PC)细胞增殖能力的影响.方法分别构建稳定表达的lv-miR-138-U、lvmiR-138-KD以及lv-NC慢病毒载体,并将慢病毒表达载体分别感染人PC细胞株PANC-1以及Capan-2;将感染后的PC细胞在体外分别采用CCK-8实验、平板克隆实验以及Ed U实验检测miR-138-5p对PC细胞增殖能力的影响;并在体内建立裸鼠皮下成瘤模型,模拟体内环境中miR-138-5p对PC细胞增殖能力的影响.结果成功地构建了重组慢病毒表达载体,并成功感染PC细胞株PANC-1和Capan-2;CCK-8、平板克隆以及Ed U实验结果均提示lv-miR-138-U组较l v-N C组增殖能力明显减弱(P<0.05),而lv-miR-138-KD组较lv-NC组增殖能力明显增强(P<0.05);体内裸鼠皮下成瘤实验显示,过表达miR-138-5p可以抑制PC细胞皮下成瘤的能力.结论 miR-138-5p可以抑制人PC细胞的增殖,为PC的治疗提供了潜在的治疗靶点.AIM To investigate the effect of micro RNA-138-5p（miR-138-5p） on the proliferation of pancreatic cancer（PC） cells.METHODS We constructed lentiviral vectors for miR-138-5p overexpression or knockdown and a negative control lentiviral vector, and transfected them into human PC cell lines PANC-1 and Capan-2. Cell counting kit-8 assay（CCK-8）, colonyforming assay and Ed U incorporation assay were employed to detect cell proliferation in vitro. The PANC-1 and Capan-2 cells were implanted subcutaneously in Balb/c nude mice to detect cell proliferation in vivo. RESULTS Lentiviral vectors were successfully constructed and transfected. CCK-8 assay, colony-forming assay and Ed U incorporation assay showed that overexpression of miR-138-5p inhibited cell proliferation compared with the negative control（P 〈0.05）, while miR-138-5p knockdown promoted cell proliferation compared with the negative control（P 〈0.05）. In addition, miR-138-5p suppressed tumor growth in the subcutaneous xenograft model of human PC cells in Balb/c nude mice.CONCLUSION Our results indicate that miR-138-5p inhibits the proliferation of PC cells, suggesting a potential new therapeutic agent for PC.

关 键 词：miR-138-5p 胰腺癌 增殖 

分 类 号：R735.9[医药卫生—肿瘤]