牙龈卟啉单胞菌rag位点基因分型与细菌毒力的关系研究  

The relationship between the rag locus genotype of Porphyromonas gingivalis and bacterial virulence

作　　者：郭兴怀 李应续[2] GUO Xing- huai LI Ying-xu(Department of Stomatology, Yangxin Third People＇s Hospital, Hubei Province 435200, Chin)

机构地区：[1]湖北省阳新县第三人民医院口腔内科,435200 [2]湖北科技学院

出　　处：《北京口腔医学》2016年第5期254-258,共5页Beijing Journal of Stomatology

摘　　要：目的探讨牙龈卟啉单胞菌rag位点基因分型与细菌毒性的关系。方法将不同rag基因位点的牙龈卟啉单胞菌W83(rag-1型)、A011/9(rag-2型)、QM220(rag-3型)和ATCC33277(rag-4型)菌株分别刺激中性多形核白细胞,未受刺激的中性多形核白细胞作为对照。采用RT-PCR检测FcγⅢb受体基因,流式细胞术检测中性多形核白细胞凋亡情况,试剂盒检测毒力因子。结果 W83组FcγⅢb基因相对表达量显著高于其他组(P<0.05),其次为QM220组,显著高于A011/9和ATCC33277组(P<0.05)。流式细胞术检测发现,ATCC33277组细胞凋亡率显著高于其他各组(P<0.05),A011/9组凋亡率显著高于W83组(P<0.05)。各基因分型组LPS、LBP、m CD14、MMP-8 MMP-9、IL-8以及IL-1β均高于对照组(P<0.05)。W83组上述指标显著高于其他各基因型组(P<0.05),QM220组次之,与A011/9、ATCC33277组比较差异有统计学意义(P<0.05)。A011/9组在LPS、LBP、m CD14方面高于ATCC33277组(P<0.05),而MMP-8、MMP-9两者差异无统计学意义(P>0.05)。结论牙龈卟啉单胞菌菌株中rag位点基因分型的细菌毒性rag-1型最强,rag-3型次之,较弱的为rag-2型和rag-4型。Objective To investigate the relationship between the Porphyromonas gingivalis （ P. g ） rag genotype and the bacterial toxicity. Methods The P. g of different rag locus strains W83 （rag-1）, A011/9 （rag-2）, QM220 （rag- 3 ） and ATC33277 （ rag-4 ） stimulated polymorphonuclear leukocyte （ PMN）, and un-stimulated PMN served as control. FcγⅢ b receptor gene was detected by RT-PCR and PMN apoptosis by flow cytometry. Results The expression of FeγⅢ b in W83 group was significantly higher than that in other groups （P 〈 0.05 ）, followed by QM220 group, and A011/9 and ATC33277 expressed the least （ P 〈 0.05 ）. The apoptosis rate of ATCC33277 group was significantly higher than other groups （P 〈0.05）, and the apoptosis rate of A011/9 group was significantly higher than that of group W83 （P 〈0.05）. LPS, LBP, mCD14, MMP-9, MMP-8, IL-8 and IL-1 of each genotype group were higher than those of control group （P 〈 0.05）. Conclusion The bacterial virulence of strain rag-1 was the strongest, followed by rag-3, and rag-2 and rag-4 was the weakest.

关 键 词：牙龈卟啉单胞菌 中性多形核白细胞 rag位点基因 细菌毒性 

分 类 号：R781.4[医药卫生—口腔医学]