一株固化珊瑚砂的巴斯德芽孢杆菌的特性研究  

Characteristics of a Bacillus pasteurii Strain for Cementation of Coral Sand

作　　者：张楠[1] 方祥位[2] 洛桑银巴 李捷[2] 欧益希 高菱悦 

机构地区：[1]后勤工程学院国防建筑规划与环境工程系 [2]后勤工程学院军事土木工程系 [3]95526部队 [4]91241部队 [5]93668部队

出　　处：《化学与生物工程》2016年第10期23-26,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(51479208;11272354);总后勤部基建营房部资助项目(CY114C022)

摘　　要：将一株具有分解尿素能力的巴斯德芽孢杆菌在不同温度、pH值、NaCl浓度下培养,通过测定脲酶活性探讨其最适生长条件;而后将最适生长条件下培养的菌悬液用于珊瑚砂固化实验,研究其固化珊瑚砂的能力。结果表明,该巴斯德芽孢杆菌最适生长条件为：温度30~33℃、pH值8.0、NaCl浓度20g·L-1;将其用于固化珊瑚砂,可使松散的珊瑚砂颗粒胶结形成具有一定强度的砂柱,但受模具限制和砂柱固化过程中渗透性的影响,砂柱各部分固化强度不一致。The optimum growth conditions of a Bacillus pasteurii strain that had the ability to decompose urea were studied by measuring the urease activity at different temperatures,pH values and NaCl concentrations.Tests of cementation of coral sand by Bacillus pasteurii cultured under the optimum growth conditions were conducted.Results indicated that,the optimum growth conditions of Bacillus pasteurii were as follows：temperature of 30~33 ℃,pH value of 8.0,and NaCl concentration of 20g·L-1.After biocementation of coral sand by Bacillus pasteurii cultured under the optimum growth conditions,loose coral sand particles were bonded to form sand columns with certain strength.Due to the limitations of test mould and permeability of sand column in cementing process,the strengths of different parts of solidified sand column were inconsistent.

关 键 词：巴斯德芽孢杆菌 珊瑚砂 固化 特性 

分 类 号：X172[环境科学与工程—环境科学] TU411[建筑科学—岩土工程]