RXRα对正常及形觉剥夺豚鼠屈光状态及眼球生长的调控  

The effect of RXRα on refractive development and ocular growth in normal and form-deprived myopic guinea pigs

作　　者：李晓清[1] 柯镜 林俊雄[1] 潘妙珍[1] 瞿佳[1] 周翔天[1] 

机构地区：[1]温州医科大学附属眼视光医院,325027

出　　处：《中华眼视光学与视觉科学杂志》2016年第9期529-535,共7页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：基金项目：国家自然科学基金（81470659）;国家自然科学基金优秀青年科学基金（81422007）;浙江省自然科学基金重点项目（LZ14H120001）

摘　　要：目的 通过对豚鼠球旁注射RXRα激动剂或抑制剂,研究RXRα在正常及形觉剥夺豚鼠屈光状态及眼球生长方面的调控作用,从而明确RXRα在屈光发育和近视形成中的作用.方法 实验研究.将245只正常3周龄体质量在100 g左右的三色豚鼠245只,采用直接抽样法分为正常组和形觉剥夺（FDM）组,2组又各分为空白对照组、溶剂组和给药组.溶剂组和给药组豚鼠每天右眼球旁分别注射DMSO或RXRα激动剂Fluorobexarotene或RXRα抑制剂HX531,连续注射4周,对侧眼不做处理.实验前和实验后2、4周分别检测屈光度和眼轴参数.正常组在实验结束后免疫荧光检测巩膜RXRα含量.采用独立样本t检验和单因素方差分析进行数据分析.结果 球旁注射Fluorobexarotene 4周后,豚鼠巩膜RXRα表达增加;球旁注射HX531 4周后,巩膜RXRα表达减少.不论注射RXRα激动剂或是抑制剂,正常组及形觉剥夺组豚鼠屈光状态和眼轴参数变化差异均无统计学意义.结论 球旁注射RXRα激动剂Fluorobexarotene或抑制剂HX531对正常及形觉剥夺豚鼠屈光发育及眼球生长无明显影响,提示单独RXRα对正常以及FDM豚鼠的屈光发育和眼球生长可能没有直接调控作用.Objective To investigate the influence of RXRα on refractive development and ocular growth,we used peribulbar injections of selective RXRα agonist and antagonist in normal and form-deprived myopic （FDM） guinea pigs.Methods In this experimental study,245 3-week-old guinea pigs weighing approximately 100 g were randomly assigned to normal and FD groups.Each group included these subgroups：untreated group,vehicle group and drug-treated group.The vehicle group and drug-treated group received peribulbar injections of DMSO,RXRα agonist Fluorobexarotene and RXRα antagonist HX531 every day for 4 weeks.Refraction and ocular biometric parameters were measured in both eyes of the individual animals before treatment and 2 and 4 weeks after treatment.RXRα expression was detected by immunofluorescence （IF） after all measurements were taken.The results were analyzed with a paired-sample t test and one-way analysis of variance.Results RXRα expression increased after peribulbar injections of RXRα agonist Fluorobexarotene.In addition,the expression of RXRα decreased after RXRα antagonist HX531 treatment.Peribulbar injection of either RXRα agonist Fluorobexarotene or RXRα antagonist HX531 did not result in statistically significant differences in refraction,vitreous chamber or axial length in the normal vision and FDM guinea pigs.Conclusion Peribulbar injection of RXRα agonist Fluorobexarotene and RXRα antagonist HX531 had no significant effect in refractive development and ocular growth in normal and FDM guinea pigs,suggesting that RXRα alone has no direct effect in the regulation of normal refractive development and form deprivation myopia in guinea pigs.

关 键 词：近视 形觉剥夺 豚鼠 维甲酸X受体 RXRα 

分 类 号：R778.11[医药卫生—眼科]