HPLC法同时测定精制冠心颗粒中5种成分的含量  

Simultaneous Determination of Five Components in Jingzhi Guanxin Granule by HPLC

作　　者：姜晖[1] 王玉[1] 付连浩 张熙洁[1] 刘晓红[1] 

机构地区：[1]唐山市工人医院,河北唐山063000

出　　处：《中国药房》2016年第30期4261-4264,共4页China Pharmacy

基　　金：河北省自然科学基金资助项目(No.C2011105042)

摘　　要：目的：建立同时测定精制冠心颗粒中丹参素、丹酚酸B、原儿茶醛、芍药苷和阿魏酸含量的方法。方法：采用高效液相色谱法。色谱柱为Zorbax Eclipse XDB-C_（18）,流动相为乙腈-甲醇-0.5%磷酸（梯度洗脱）,流速为1.0 ml/min,检测波长为280 nm（丹参素、原儿茶醛、丹酚酸B）、230 nm（芍药苷）、320 nm（阿魏酸）,柱温为30℃,进样量为1.0μl。结果：丹参素、原儿茶醛、丹酚酸B、芍药苷和阿魏酸检测质量浓度线性范围分别为1.19-478.34μg/ml（r=0.999 9）、0.11-44.93μg/ml（r=0.999 9）、7.49-995.20μg/ml（r=0.999 7）、0.95-379.39μg/ml（r=0.999 9）、0.01-3.12μg/ml（r=0.999 5）;定量限分别为1.91、0.36、150.00、2.74、0.10 ng,检测限分别为0.96、0.10、45.00、1.52、0.03 ng;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为98.06%-99.47%（RSD=0.52%,n=6）、98.01%-99.49%（RSD=0.70%,n=6）、98.44%-99.45%（RSD=0.37%,n=6）、96.94%-100.71%（RSD=1.27%,n=6）、95.44%-100.44%（RSD=1.90%,n=6）。结论：该方法操作简单、结果准确,适用于同时测定精制冠心颗粒中丹参素、原儿茶醛、丹酚酸B、芍药苷和阿魏酸的含量。OBJECTIVE：To establish a method for simultaneous determination of danshensu,salvianolic acid B,protocatechuic aldehyde,paeoniflorin and ferulic acid in Jingzhi guanxin granule. METHODS：HPLC was performed on the column of Zorbax Eclipse XDB-C_（18） with mobile phase of acetonitrile-methanol-0.5% H_3PO_4（gradient elution）at a flow rate of 1.0 ml/min,the detection wavelength was 280 nm（for danshensu,protocatechuic aldehyde,salvianolic acid B）,230 nm（for ferulic acid）and 320 nm（for paeoniflorin）,column temperature was 30 ℃,injection volume was 10 μl. RESULTS：The linear range was 1.19-478.34 μg/ml for danshensu（r=0.999 9）,0.11-44.93 μ g/ml for protocatechuic aldehyde（r=0.999 9）,7.49-995.20 μ g/ml for salvianolic acid B（r=0.999 7）,0.95-379.39 μg/ml for paeoniflorin（r=0.999 9）and 0.01-3.12 μg/ml for ferulic acid（r=0.999 5）;the limits of quantitation were 1.91 ng,0.36 ng,150.00 ng,2.74 ng and 0.10 ng,limit of detection were 0.96 ng,0.10 ng,45.00 ng,1.52 ng and 0.03 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.06%-99.47%（RSD=0.52%,n=6）,98.01%-99.49%（RSD=0.70%,n=6）,98.44%-99.45%（RSD=0.37%,n=6）,96.94%-100.71%（RSD=1.27%,n=6）and 95.44%-100.44%（RSD=1.90%,n=6）. CONCLUSIONS：The method is simple and accurate,and suitable for the simultaneous determination of danshensu,salvianolic acid B,protocatechuic aldehyde,paeoniflorin and ferulic acid in Jingzhi guanxin granule.

关 键 词：含量测定 高效液相色谱法 丹参素 原儿茶醛 丹酚酸B 芍药苷 阿魏酸 

分 类 号：R917[医药卫生—药物分析学]