极端嗜盐古菌Natrinema sp.J7-2的转录组学研究  

Transcriptome characteristics of Natrinema sp.J7-2 analyzed with RNA-sequencing technology

作　　者：梅运军[1] 邓威[2] 张顺喜[1] 胡纯[1] 沈萍[3] Yunjun Mei Wei Deng Shunxi Zhang Chun nu Ping Shen(School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China College of Life Sciences, Wuhan University, Wuhan 430072, China)

机构地区：[1]武汉轻工大学化学与环境工程学院,湖北武汉430023 [2]武汉轻工大学生物与制药工程学院,湖北武汉430023 [3]武汉大学生命科学学院,湖北武汉430072

出　　处：《生物技术》2016年第5期463-468,共6页Biotechnology

基　　金：湖北省教育厅科学研究计划资助项目("嗜盐古菌Natrinema sp.J7-2与其噬菌体生态适应的机制研究";No.D20151704)

摘　　要：[目的]从转录组的角度研究极端嗜盐古菌Natrinema sp.J7-2的盐适应性。[方法]利用Illumina Hi Seq 2000双末端测序技术对生长在不同盐浓度下(15%Na Cl、25%Na Cl、30%Na Cl)的Natrinema sp.J7-2的转录组测序分析,并对差异表达基因进行了GO与KEGG富集。[结果]转录组测序结果表明,培养在3种不同盐浓度下的Natrinema sp.J7-2差异表达基因主要富集到氨基酸代谢、卟啉与叶绿素代谢两条途径上,氨基酸代谢主要涉及谷氨酸、天冬氨酸、丙氨酸代谢;在3组盐培养物中,有1 320个基因存在差异,其中83个共同差异表达的基因。[结论]通过转录组分析揭示Natrinema sp.J7-2菌株主要通过调控氨基酸代谢与能量代谢来适应不同盐环境。[ Objective]To explore the salt adaptation of Natrinema sp. J7 -2 based on transcriptomics sequencing. [ Methods ] Illumina HiSeq 2000 pair - end sequencing technology was employed to analyze the Natrinema sp. J7 - 2 grown under different salt concentrations （15 % NaC1,25 % NaC1 and 30% NaC1）, then the differential expression analysis was carried out, and then GO and KEGG of differentially expressed genes were enriched. [ Results ] The differentially expressed genes were mainly enriched in amino acids metablism, porphyrin and chlorophyll metabolism; amino acids metabolism mainly involved glutamate, aspartic acid and alanine. And there were 1 320 differentially expressed genes and only genes 83 commonly differentially expressed genes among cells incubated in 15% ,25% and 30% NaC1 media. [ Conclusion] Natrinema sp. J7 -2 cells regulated amino acids metabolism and energy metabolism to adapt different salinity environments.

关 键 词：RNA-SEQ 转录组学 盐适应 嗜盐古菌 

分 类 号：Q93-31[生物学—微生物学] Q935