Nestin-LacZ转基因小鼠脊髓损伤动物模型的建立及评价  

Establishment and evaluation of Nestin-LacZ transgenic mouse model of spinal cord injury

作　　者：秦毅[1,2] 叶记超[2] 王鹏[2] 黄霖[2] 唐勇[2] 王素伟[1] 沈慧勇[2] 

机构地区：[1]广东省珠海市人民医院脊柱骨病科,519000 [2]中山大学孙逸仙纪念医院中山大学脊髓损伤研究所,广州510120

出　　处：《中国矫形外科杂志》2016年第20期1888-1891,共4页Orthopedic Journal of China

基　　金：国家自然科学基金项目(编号:U1301223);中国博士后科学基金面上资助项目(编号:2015M570741);中国博士后科学基金特别资助项目(编号:2016T90820);广东省科技计划项目资助(编号:2016A020225001);广东省医学科学技术研究基金项目资助(编号:A2016233);广东省中医药局科研项目资助(编号:20161233)

摘　　要：[目的]建立标准的Nestin-LacZ转基因小鼠脊髓损伤动物模型及评价体系。[方法]采用NestinLac Z转基因小鼠18只,随机分为三组,实验组2个,对照组1个。2个实验组均采用自行研制的啮齿类动物脊髓损伤模型打击器,质量为10 g的打击棒,A实验组用接触直径为2.0 mm的打击棒杆头压迫,压迫时间为3 min(A组,6例);B实验组按照实验设定的高度10.0 mm实施打击(B组,6例);对照组仅行椎板切除,不打击(C组,6例)。分别于术前,术后第1、2、3、4、5、6周进行转基因小鼠BBB运动评分,取出脊髓组织后,行脊髓组织HE染色。[结果]Nestin-LacZ转基因小鼠不同的脊髓损伤模型在各个时间点上BBB评分差异有统计学意义(P<0.05),A组在脊髓损伤造模术后2周,后肢功能开始恢复,而B组至术后6周均无后肢功能恢复,对照组无功能障碍。[结论]啮齿类动物脊髓损伤模型打击器采用持续慢性压迫脊髓可成功建立Nestin-Lac Z转基因小鼠稳定、理想的急性脊髓损伤模型。[ Objective] To establish a standard Nestin -LacZ transgenic mouse model of spinal cord injury and the related evaluation system. [Method]A total of 18 Nestin -LacZ transgenic mice were randomly divided into two experimental groups and one control group. In one experimental group （group A, n = 6）, meanwhile, a self - developed impacting rod （ 10 g） for es- tablishment of the spinal cord injury model in rodents was applied, and compression was performed with the tip of the impacting rod （ contact diameter 2.0 mm） for 3 rain. In the other experimental group （ group B, n = 6）, meanwhile, a self - developed im- pacting rod （10 g） for establishment of the spinal cord injury model in rodents was applied, and impacting was performed at a height of 10.0 mm. The mice in the control group （group C ,n =6） underwent laminectomy alone. Before injury and at 1,2,3, 4,5 ,and 6 weeks after injury, the Basso- Beattie- Bresnahan （BBB） scores were measured; the spinal cord tissues were col- lected for histological observation and then subjected to HE staining. [ Result] BBB scores showed significant differences be- tween the Nestin - LacZ transgenic mice with different types of spinal cord injury at all time points （ P 〈 0.05）. The mice in group A had functional recovery in hind limbs at 2 weeks after injury,while those in group B had no functional recovery in hind limbs throughout 6 weeks after injury;the mice in group C showed no dysfunction. [ Conclusion] Continuous compression of the spinal cord with the self - developed impactor for the establishment of the spinal cord injury model in rodents can successfully establish a stable and ideal Nestin - LacZ trans- genic mouse model of acute spinal cord injury.

关 键 词：脊髓损伤 nestin—LacZ转基因小鼠 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]