唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究  

Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens

作　　者：薛龙[1,2] 王立鹤[1,2] 聂红兵[3,4] 周海静[3,4] 郭曼丽[3,4] 康永杰[1,2] 杨兰[1] XUE Long WANG Li - he NIE Hong- bing ZHOU Hai -jing GUO Man - li KANG Yong -jie YANG Lan(Department of Oral and Maxillofacial Surgery, The Second Hospital of Lanzhou University, Lanzhou 730000, China Key Laboratory of Oral Diseases of Gansu Provincial, Northwest University for Nationalities, Lanzhou 730000, China Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, China School of Stomatology, Lanzhou University, Lanzhou 730000, China)

机构地区：[1]兰州大学第二医院口腔颌面外科,甘肃兰州730030 [2]兰州大学口腔医学院 [3]西北民族大学甘肃省口腔疾病研究重点实验室培育基地 [4]西北民族大学口腔医学国家民委重点实验室

出　　处：《口腔医学研究》2016年第10期1042-1046,共5页Journal of Oral Science Research

基　　金：甘肃省自然科学基金(编号:1506RJZA276);国家民委科研基金(编号:14XBZ022)

摘　　要：目的:研究唾液乳酸杆菌w22a的潜在益生性和与种植体周围炎致病菌:牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、中间普氏菌(Pevotellaintermedia,Pi)、具核梭酸杆菌(Fusobacteriumncleatum,Fn)的体外作用,为乳酸杆菌w22a作为益生菌应用于种植体周围炎的防治提供实验依据。方法::通过检测w22a代谢产物成分、w22a对抑菌物质的耐受性等,初步探讨其口腔益生特性;并通过自聚和共聚实验、抑菌实验研究唾液乳酸杆菌w22a和种植体周围炎致病菌Pg、Pi、Fn的体外作用。结果:发现唾液乳酸杆菌w22a可产生有机酸、过氧化氢等抑菌物质,并能产生胞外多糖;w22a能够耐受一定浓度的抗生素和溶菌酶;w22a在体外有自聚集和与Pg、Pi、Fn发生共聚集的能力;其代谢产物对于Pg、Pi、Fn的生长具有不同程度的抑制作用。结论:唾液乳酸杆菌w22a能够对Pg、Pi、Fn产生生长抑制作用,具有口腔益生性,有望作为益生菌用于种植体周围炎的防治。Objective：To evaluate whether the oral administration of Lactobacillus salivarius w22 acould alleviate the peri-implantitis pathogens in subgingival plaque,such as Porphyromonas gingivalis（Pg）,Pevotella intermedia（Pi）,and Fusobacteriurn nuclearum（Fn）in vitro.Methods：The antagonistic growth effects of Lactobacillus salivarius as well as their probiotic on periodontal pathogens,including Pg,Pi and Fn,were tested using drug resistance experiment,agglutination experiments,and antibacterial experiments.Results：The results revealed that Lactobacillus salivarius w22 acould produce antibiotics such as organic acid,hydrogen peroxide and EPS.Strain w22 acould resist lysozyme and antibiotics at certain concentration.In addition,auto-agglutination and agglutination with other pathogens could be observed.The fermentation of w22 a inhibited the growth of pathogens to varied degrees.Conclusion：Lactobacillus salivarius w22 awas a potential probiotic strain to inhibit the growth of Pg,Pi and Fn in vitro.

关 键 词：唾液乳杆菌 益生菌 种植体周围炎 

分 类 号：R783.6[医药卫生—口腔医学]