膜联蛋白A2对人结肠癌细胞SW620侵袭迁移的影响  

Effects of Annexin A2 on the Invasion and Migration of Human Colon Cancer Cell Line SW620

作　　者：蒋丽[1] 张桂英[2] 张晓梅[2] 李辉[1] 穆仪冰[1] 

机构地区：[1]湖南省长沙市第四医院消化内科,长沙410006 [2]中南大学湘雅医院消化内科,长沙410008

出　　处：《肿瘤预防与治疗》2016年第5期249-253,共5页Journal of Cancer Control And Treatment

基　　金：湖南省科技厅资助项目(编号2011FJ3012)

摘　　要：目的:研究膜联蛋白A2(ANXA2)对人结肠癌细胞SW620侵袭迁移的影响。方法:采用siRNA技术沉默ANXA2在SW620细胞中的表达,实验分为干扰组、对照组和野生组,RT-PCR法检测各组ANXA2 mRNA水平,Western blot法检测各组ANXA2、纤维蛋白溶酶(PL)、基质金属蛋白酶-1(MMP-1)、血管内皮生长因子(VEGF)蛋白的表达,Transwell侵袭实验检测各组SW620细胞穿膜能力。结果:干扰组与对照组、野生组相比,ANXA2 mRNA水平、ANXA2、PL、MMP-1、VEGF蛋白表达及SW620穿膜细胞数差异均有统计学意义(P<0.05),而对照组和野生组相比上述各指标均无统计学差异。ANXA2蛋白和PL、MMP-1、VEGF蛋白水平、穿膜细胞数呈正相关(P<0.05)。结论:沉默ANXA2基因后可能通过下调PL、MMP-1及VEGF蛋白的表达,抑制SW620细胞侵袭迁移能力起到抗肿瘤作用。Objective： To investigate the effects of Anrlexin A2 （ANXA2） on cell invasion and migration of human colon cancer cell line SW620. Methods： The expression of ANXA2 in SW620 cells was silenced by siRNA technique. The experiment was divided into three groups ： interference group, control group and wild group. The levels of mRNA of ANXA2 in each group were detected by RT-PCR. The expressions of ANXA2, plasmin（PL） , matrix metalloproteinase-1 （MMP-1） and vascular endothelial growth factor （VEGF） protein in each group were detected by Western blot method. The invasion and migration ability of SW620 cells in each group were detected by Transwell invasion assay. Results： Compared with the control group and the wild group, the level of ANXA2 mRNA, the expressions of ANXA2, PL, MMP-1, VEGF proteins and the numbers of SW620 transmembrane cells in the interference group were statistically significant higher or lower （ P 〈 0.05 ）. And there were no significance in the above indicators between the control group and the wild group. The correla- tions between the expression of ANXA2 protein and the expressions of PL, MMP-1, and VEGF proteins and the numbers of transmembrane cells were positively correlated （ P 〈 0.05 ）. Conclusion ： Silencing of ANXA2 gene may play a role in the anti-tumor effect by down-regulating the expressions of PL, MMP-1 and VEGF proteins and inhibiting the invasion and migration of SW620 cells.

关 键 词：膜联蛋白A2 结肠癌 纤维蛋白溶酶 基质金属蛋白酶-1 血管内皮生长因子 

分 类 号：R735.35[医药卫生—肿瘤]