节杆菌(Arthrobacter sp.)CN2对对硝基苯酚的趋化性研究  

Chemotaxis of Arthrobacter sp. CN2 towards p-nitrophenol

作　　者：乔铖 任磊[1] 贾阳[1] 樊双虎[1] 王俊欢[1] 闫艳春[1] 

机构地区：[1]中国农业科学院研究生院,北京100081

出　　处：《农业环境科学学报》2016年第10期1945-1952,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(31170119;31540067)

摘　　要：利用已获取的对硝基苯酚降解菌CN2,以对硝基苯酚为底物,通过游动平板趋化性、土壤趋化性实验以及毛细管趋化性实验对该菌株的趋化特性进行了研究,同时通过模拟土壤原位修复探究了菌株在实际应用中的效果。高效液相色谱检测结果表明,72 h内菌株CN2对对硝基苯酚的降解率大于99%。CN2在平板趋化性与土壤趋化性实验中均表现出对对硝基苯酚的趋化性特征,毛细管趋化性实验中,当对硝基苯酚浓度在一定范围内(5~800 mg·L^(-1))时,菌株的趋化性与对硝基苯酚浓度呈正相关,当其浓度高于800mg·L^(-1)时,菌株的趋化性逐渐受到抑制。模拟土壤原位修复实验的结果表明,菌株CN2在未灭菌土壤中的对硝基苯酚降解速率高于灭菌后土壤中的降解速率,在14 d内其降解率可达95%。研究表明,该菌株对环境具有良好的适应性,其对污染环境修复具有应用价值与潜力。Microbial chemotactic systems are critical for bacteria to sense chemicals in the surrounding medium, which enable them to move towards or away from certain chemotant. Strain Arthrobacter sp. CN2, which could efficiently degrade p-nitrophenol,was used in swarm plate assay, soil chemotaxis assay and capillary chemotaxis assay with p-nitrophenol as the sole substrate, respectively. In the meanwhile, a test of in situ soil assay was carried out in order to assess the application performance of CN2. High Performance Liquid Chromatography（HPLC）test revealed the ability of strain CN2 to degrade over 99% of p-nitrophenol within 72 h. A series of chemotaxis assays indicated that strain CN2 had obvious chemotaxis toward p-nitrophenol. Specifically in the capillary assay, the strain was positively chemotactic to pnitrophenol within a certain range of concentrations（5～800 mg·L-1）, but was gradually inhibited when the concentration of p-nitrophenol was higher than 800 mg·L-1. Furthermore, test of in situ soil assay showed a higher degradation efficiency in unsterilized soil than in sterilized soil where the degradation rate could reach up to 95% within 14 days. Our data revealed an excellent adaptability of CN2 to the environment and claimed the potential application in environmental remediation of pollutants.

关 键 词：节杆菌 对硝基苯酚 趋化性 环境修复 

分 类 号：X171.5[环境科学与工程—环境科学]