miR-577在胃癌中的表达及对SGC-7901细胞侵袭的影响  

Expression of miR-577 and Its Role for Cell Invasion in Human Gastric Carcinoma

作　　者：何银平[1] 叶景旺[2] 

机构地区：[1]第三军医大学大坪医院信息资料科,重庆400042 [2]第三军医大学大坪医院野战外科研究所胃结直肠外科

出　　处：《医学研究杂志》2016年第10期95-98,共4页Journal of Medical Research

摘　　要：目的研究微小RNA577（microRNA-577，miR-577）在人胃癌（gastric cancer，GC）组织内的表达情况及对肿瘤细胞侵袭能力的影响。方法选择2013年1月～2015年3月于第三军医大学大坪医院普通外科收治并行手术切除的70例胃癌与对应癌旁组织。通过qRT—PCR方法检测miR-577在组织中的相对表达量，分析miR-577的临床病理意义；采用人工合成的miR-577模拟物（miR-577mimics）转染人胃癌SGC-7901细胞，并检测SGC-7901细胞过表达miR-577后侵袭能力及miR-577下游潜在靶点B—cateninmRNA及蛋白的表达变化。结果MiR-577在胃癌组织中表达显著下调（P=0．000）并与与淋巴结转移（P=0．003）、高TNM分期（P=0．006）等不良临床特征呈正相关；过表达miR-577能够显著抑制SGC-7901细胞侵袭能力（P=0．000）及细胞内β-catenin的表达水平。结论miR-577在胃癌中低表达，并可能通过下调β-catenin的表达来抑制胃癌细胞的侵袭。Objective To identify the level of microRNA - 577 and its effect for cell invasion in human gastric carcinoma（GC）. Methods The level of miR - 577 in 70 paired GC and matched non - tumor tissues collecting between January 2011 and March 2015 was detected by qRT - PCR. We then transfected the miR -577 mimics into SGC -7901 cells. Cell invasion ability was measured by transwell assay, qRT - PCR and western - blot were used to detect the expression of β - catenin, a potential target of miR - 577, in transfected SGC - 7901 ceils. Results miR - 577 was low expression in gastric cancer tissues and correlated with lymphatic metastasis（ P = 0. 003） and advanced TNM stage（ P = 0. 006）. Transfection of miR - 577 mimics could suppress cell invasion （ P = 0. 000） and inhibit the expression of β - catenin （ P = 0. 000）. Conclusion miR - 577 was low expression in GC tissues, and miR - 577 could suppress GC invasion through down - regulating ~ - catenin.

关 键 词：MicroRNA-577 胃癌 β—catenin侵袭 

分 类 号：R73-3[医药卫生—肿瘤]