FLT3抑制剂AC220抑制眼新生血管的作用及机制  

Inhibitory effect of FLT3 inhibitor AC220 on ocular neovascularization and its mechanisms

作　　者：高誉硕 谢冰[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院眼科,上海200025

出　　处：《上海交通大学学报（医学版）》2016年第10期1440-1444,1450,共6页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的·观察FMS-样酪氨酸激酶3(FLT3)与眼新生血管(NV)的关系,探讨FLT3抑制剂AC220对抗眼NV的作用机制。方法·建立小鼠氧诱导视网膜病变(OIR)模型和脉络膜新生血管(CNV)模型,采用免疫荧光法和酶联免疫吸附试验观察FLT3在OIR和CNV小鼠模型中的表达。在玻璃体腔内注射AC220,采用免疫荧光法观察其对眼NV的作用和对树突状细胞(DC)数目的影响。结果·FLT3在OIR和CNV模型小鼠视网膜的表达较对照组明显升高。玻璃体腔内注射AC220能有效减少OIR和CNV模型小鼠中眼NV的面积,并减少视网膜中DC的数目。结论·FLT3与眼NV密切相关。AC220具有抑制NV生成和调节DC数目的作用,有望成为临床抗NV治疗的辅助药物。Objective·To investigate association between FMS-like tyrosine kinase 3 （FLT3） and ocular neovascularization （NV） and explore the mechanisms of action of a novel FLT3 inhibitor AC220 in inhibiting ocular NV. Methods·A mouse model of oxygen induced retinopathy （OIR） and a mouse model of choroidal neovascularization （CNV） were constructed. FLT3 expressions in two models were detected by immumofluorescence method and ELISA. AC220 was intravitreally administrated and the effects of AC220 on ocular NV and the number of dendritic cell （DC） were investigated by immumofluorescence method. Results·The level of retinal FLT3 was significantly higher in the OIR and CNV groups than in the control group. Intravitreal administration of AC220 significantly reduced the ocular NV area in OIR and CNV groups and decreased the number of DC in retina. Conclusion·FLT3 is closely associated with ocular NV. AC220 can inhibit the development of NV, adjust the number of DC, and is a potential adjuvant medicine for anti-NV treatment.

关 键 词：FMS-样酪氨酸激酶3 AC220 眼新生血管 脉络膜新生血管 树突状细胞 

分 类 号：R774[医药卫生—眼科]