ClC-3与BK离子通道在胶质瘤细胞侵袭性生长中的作用研究  

The roles of ClC-3 and BK ion channel in glioma cells invasion

作　　者：曹敬正 卢培刚[1] 董元[1] 李博[1] 梁媛[1] 郝振强[1] 李育鑫[1] 

机构地区：[1]济南军区总医院神经外科,山东济南250031

出　　处：《中华神经外科疾病研究杂志》2016年第5期389-392,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的观察3型氯离子通道(Cl C-3通道)与大电导钙激活K+通道(BK通道)在胶质瘤细胞中的表达;研究Cl C-3离子通道与BK离子通道在胶质瘤细胞侵袭性生长中的作用。方法 Western blot实验检测Cl C-3与BK通道分别在C6及U251胶质瘤细胞中的蛋白表达,免疫荧光技术检测两种通道蛋白在胶质瘤细胞中的形态学分布情况;采用Transwell侵袭实验检测应用Cl C-3通道特异性阻滞剂氯代毒素(Cl TX)以及BK通道特异性阻滞剂伊比蝎毒素(IBTX)后胶质瘤细胞侵袭能力的变化情况。结果 U251及C6胶质瘤细胞系均高度表达Cl C-3离子通道,而BK离子通道在两种胶质瘤系中的表达相对较低;应用Cl TX后U251细胞的侵袭性显著降低(P<0.05),且呈剂量依赖关系;应用IBTX后U251细胞的侵袭性无明显变化。结论 Cl C-3离子通道在胶质瘤侵袭性生长发挥重要作用,可作为未来治疗胶质瘤的新靶标。Abstract Objective The expressions of voltagegated chloride channel-3 （ClC-3 channel） and big mediate conductance Ca2＋ activated K＋ channel （BK channel） in glioma cells are observed and the roles of ClC-3 and BK ion channel on invasion of glioma cells are investigated.MethodsThe expressions of ClC-3 and BK ion channel in two glioma cell lines （C6 and U251） were evaluated by Western blot; immunofluorescence staining was used to detect the distribution of the two channels in C6 and U251 glioma cell lines; Transwell invasion assay was used to determine the effects of chlorotoxin （ClTX, a ClC-3 inhibitor） and iberiotoxin （IBTX, a BK inhibitor） on the invasion of the two glioma cell lines.ResultsU251 and C6 glioma cell lines were highly expressed in ClC-3 ion channel, but the expression of BK ion channel in two glioma lines was relatively lower; U251 glioma invasion was decreased significantly after the application of ClC-3 specific blocker chlorotoxin in a dosedependent manner, but the BK channel specific blocker iberiotoxin showed no obvious effect in U251 glioma invasion.ConclusionClC-3 ion channels play an important role in the invasive growth of glioma, suggesting that ClC-3 ion channels might be a new target for the future therapy of glioma.

关 键 词：神经胶质瘤 ClC-3与BK离子通道 侵袭 靶向治疗 

分 类 号：R739.41[医药卫生—肿瘤]