CCR2抑制剂RS504393对小鼠短暂性局灶性脑缺血的保护作用研究  

Protective Effects of CCR2 Inhibitor RS504393 on Transient Focal Cerebral Ischemia in Mice

作　　者：王涛[1] 方伟蓉[2] 胡锦[1] 

机构地区：[1]复旦大学附属华山医院神经外科,200040 [2]中国药科大学生理学系,210009

出　　处：《中国临床神经科学》2016年第5期519-522,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的探讨CCR2抑制剂RS504393对小鼠脑缺血的治疗作用。方法用野生型小鼠(C57BL/6)40只构建短暂性局限性脑缺血模型,随机分为假手术组(麻醉后,分离暴露血管,但不使用线栓堵塞大脑中动脉);模型组(给予等容积的生理盐水,腹腔注射);高剂量组(RS504393 2 mg·kg^(-1),腹腔注射);低剂量组(RS504393 1 mg·kg^(-1),腹腔注射)。使用TTC染色计算梗死体积,行为学方法评价,脑组织含水量检测等技术方法分析CCR2抑制剂RS504393对小鼠脑缺血的治疗作用。结果与模型组比较,高剂量组神经功能显著改善、脑梗死体积百分比降低、脑水肿减轻;低剂量组对该模型也有一定改善作用。结论在小鼠脑缺血早期给予CCR2抑制剂RS504393对缺血脑组织有保护作用。Aim To investigate the therapeutic effect of CCR2 inhibitor RS504393 on cerebral ischemia in mice. Methods The model of transient focal cerebral ischemia was constructed with wild type mice（C57BL/6）. In order to analyze the therapeutic effects of CCR2 inhibitor RS504393 on cerebral ischemia in mice, many different methods including TTC staining, neurobehavior test, and brain tissue water content detection were used. Results Compared with the model control group, RS 504393 intraperitoneal injection（ip） high dose group（2 mg·kg^（-1） improved the neurological function, reduced the percentage of infarction volume and attenuated cerebral edema. RS504393 low dose group（0.1 mg·kg^（-1）） of the model was also improved. Conclusion CCR2 inhibitor RS 504393 had protective effect in the early stage of cerebral ischemia in mice.

关 键 词：脑缺血 CCR2抑制剂 单核细胞来源的巨噬细胞 免疫干预 

分 类 号：R743[医药卫生—神经病学与精神病学]