c-myc蛋白表达水平与c-myc基因易位相关性分析及其临床应用  

Analysis of the correlation between c-myc expression and c-myc translocation and the clinical significance

作　　者：黄文亭[1] 郭蕾[1] 郑波[1] 吕宁[1] 

机构地区：[1]国家癌症中心/中国医学科学院北京协和医学院肿瘤医院病理科,北京100021

出　　处：《临床与实验病理学杂志》2016年第10期1110-1112,1117,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：首都临床特色应用研究(Z151100004015121);中国癌症基金会北京希望马拉松基金(LC2013B40;LC2014L13)

摘　　要：目的探讨c-myc蛋白表达与c-myc基因易位的相关性,旨在寻找适用于临床c-myc蛋白的界定值,以有效判断c-myc基因易位状态。方法采用免疫组化法与荧光原位杂交(fluorescence in situ hybridization,FISH)法检测293例弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中c-myc蛋白表达水平与c-myc基因易位状态,分析两者的相关性。结果 293例DLBCL中,30例(10.2%)发生c-myc基因易位,其中29例c-myc蛋白阳性细胞百分率>40%,1例c-myc蛋白阳性细胞百分率为40%,而c-myc蛋白阳性细胞百分率<40%者均未发生c-myc基因易位。随着c-myc蛋白表达水平的升高,c-myc基因易位的发生率也随之升高。结论 c-myc蛋白高表达与c-myc基因易位密切相关。c-myc蛋白可用于DLBCL中c-myc基因易位的筛选,建议对c-myc蛋白阳性细胞百分率>40%者行FISH检测以进一步明确有无c-myc基因易位。Purpose To study the correlation between c-myc expression and c-myc translocation, in order to find out an appropriate cut-off level of c-myc expression for estimation of c-myc translocation in clinical practice. Methods In 293 cases of diffuse large B- cell lymphoma （DLBCL）, e-myc expression was observed by immunohistochemistry （IHC） and its translocation was detected by fluo- rescence in situ hybridization （FISH）. And the correlation between c-myc expression and translocation was subsequently analyzed. Re- sults In 293 cases of DLBCL, c-myc translocation was detected in 30 （ 10. 2% ） of cases. 29 of them were c-myc expression 〉 40% , and the remained one was 40%. While, none of the cases with c-myc expression 〈 40% had c-myc translocation. Furthermore, the higher c-myc expressed, the more c-myc translocation occurred. Conclusion C-myc overexpression is obviously correlated with c-myc translocation. It is useful to detect the level of e-mye expression in DLBCL so that to evaluate whether it had c-myc translocation. It is suggested that it should be further examined c-myc translocation condition by FISH when its level of c-myc expression is higher than 40%.

关 键 词：淋巴瘤弥漫大B细胞淋巴瘤 C-MYC 免疫组织化学 荧光原位杂交 

分 类 号：R733.4[医药卫生—肿瘤]