IgA／EBV—VCA阳性人群和鼻咽癌患者的EB病毒潜伏膜蛋白2特异性细胞免疫及EBVDNA载量检测  

Detection of EBV-LMP2-specific cell responses and EBV DNA copies in IgA/EBV-VCA positive individuals and patients with nasopharyngeal carcinoma

作　　者：谭珍连[1] 王勇利 麦稚平 廖建 王湛[4] 詹少兵[4] 杜海军[4] 

机构地区：[1]广西梧州市疾病预防控制中心,643200 [2]广西自治区人民医院 [3]广西苍梧鼻咽癌防治所,643200 [4]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,102206

出　　处：《中华实验和临床病毒学杂志》2016年第5期493-495,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家973项目（2011CB504800）;国家863项目（2012AA02A404）;环境与病毒肿瘤学北京市重点实验室课题项目（EV02012021）

摘　　要：目的探讨IgA／VCA抗体滴度与EBVLMP2特异性细胞免疫水平和EBVDNA拷贝数关系。方法免疫酶法检测普查样本IgA／VCA抗体滴度；选取IgA／VCA抗体阳性人群和治疗前鼻咽癌患者，通过γ-干扰素Elispot和EBVDNA荧光定量试剂盒检测LMP2特异性细胞免疫水平和血浆中的EBVDNA载量。结果普查广西苍梧县城自然人群共1233人，其中80人IgA／VCA抗体阳性，阳性率为6．0％。对其中的72例IgA／VCA抗体阳性人群和20未治疗的鼻咽癌患者进行了LMP2特异性细胞免疫和EBVDNA检测，随着抗体滴度的升高，LMP2特异性细胞免疫反应水平降低，而血浆中EBVDNA拷贝数有随IgA／VCA抗体滴度升高而增加的趋势。结论EBV—LMP2特异性细胞免疫水平和EBVDNA拷贝数可能与IgA／VCA抗体滴度相关。Objective To investigate the correlation between EBV-LMP2-specific cell responses, EBV DNA copies and titers of IgA antibody to viral capsid antigen （IgA/VCA）. Methods IgA/VCA antibody were tested with immune-enzyme method. Both of plasma and lymphocytes were respectively separated from IgA/VCA-positive individuals and patients with nasopharyngeal carcinoma （NPC） without treatment. EBV DNA copies and LMP2-specifie cell responses were detected by interferon-gamma Elispots assay and fluorescent quantitative PCR method. Results IgA/VCA antibody in 80 of 1 233 individuals was positive in Cangwu county, and the positive rate is 6%. EBV-LMP2-specific cell responses and EBV DNA copies were detected in 72 of 80 IgA/VCA positive individuals and patients with NPC. EBV DNA copies in plasma were increased with rise of titers of IgA/VCA antibody, while EBV-LMP2-specific cell responses were declined. Conclusion EBV-LMP2-specific cell responses and EBV DNA copies are probably associated with titers of IgA/VCA antibody.

关 键 词：IgA/EBV—VCA 潜伏膜蛋白2 细胞免疫 EBV DNA 

分 类 号：R739.63[医药卫生—肿瘤]