GC-MS法分析“发汗”对续断挥发性成分的影响  

Effect of “Fahan” on volatile constituents in Dipsaci Radix by GC-MS

作　　者：杨莹[1] 杜伟锋[2] 岳显可[2] 朱涛[2] 来平凡[1] 葛卫红[2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江中医药大学中药炮制技术研究中心,浙江杭州311401

出　　处：《中成药》2016年第10期2222-2226,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年科学基金项目(81303224)

摘　　要：目的通过GC-MS法分析＂发汗＂对续断Dipsacus asper Wall.ex Henry挥发性成分的影响。方法水蒸气蒸馏法提取续断乙醚萃取物挥发油后,GC-MS法鉴定其成分,面积归一化法计算各成分相对含有量。结果续断在＂发汗＂前后均鉴定出28种成分,其中26种为共有成分。＂发汗＂前未检测出9,12,15-十八碳三烯酸甲酯和9-亚甲基-9H-芴,而＂发汗＂后未检测出顺-13-十八碳烯酸和十七烷醇。结论 ＂发汗＂后续断中共有成分的含有量均有明显变化,变化范围为30.81%-99.34%。AIM To analyze the effect of ＂Fahan＂（ herbs piled and naturally dried） on volatile constituents in Dipsacus asper Wall. ex Henry by GC-MS. METHODS After the volatile oil in D. asper ethereal extract was extracted by steam distillation,its constituents were identified by GC-MS,then the relative contents of various constituents were calculated by area normalization method. RESULTS Before and after ＂Fahan＂,twenty-eight constituents were identified in D. asper,twenty-six of which were common components. Methyl 9,12,15-octadecatrienoate and 9-methylene-9H-fluorene were not detected before ＂Fahan＂,while 11-octadecenoicacid,（ 11Z）-and 1-heptadecanol were not detected after ＂Fahan＂. CONCLUSION After ＂Fahan＂,the contents of common components in D. asper all show obvious changes within the ranges of 30. 81%- 99. 34%.

关 键 词：续断 “发汗” 挥发性成分 GC-MS 

分 类 号：R284.1[医药卫生—中药学]