黑曲霉菌发酵降解茶皂素的条件优化  

Optimization of fermentation conditions for tea saponin degradation by Aspergillus niger

作　　者：覃妍[1] 肖瑜[1] 黄浦[1] 刘以清 易茗[1] 朱芸[1] 

机构地区：[1]桂林理工大学广西岩溶地区水污染控制与用水安全保障协同创新中心,桂林541004

出　　处：《安徽农业大学学报》2016年第5期698-703,共6页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(51569008);广西自然科学基金(2015GXNSFAA139240);广西矿冶与环境科学实验中心(KH2012ZD004)共同资助

摘　　要：原料以茶皂素的降解率为评价指标,利用黑曲霉菌液态发酵降解茶皂素。先通过单因素实验确定最佳的发酵时间、发酵温度、培养基初始pH、摇床转速、接种量,再通过曲面响应实验对发酵条件进行优化。结果表明,影响降解率显著性次序为:发酵温度>培养基pH>接种量>发酵时间>摇床转速,得到最佳的优化条件为:发酵时间8 d,发酵温度38.7℃,培养基初始pH为6.14,摇床转速为125 r·min^(-1),接种量1.25%。在以上最佳条件下进行实验,得到降解率为(79.97±0.82)%,与理论值80.56%吻合。In this study, we used liquid fermentation to degrade tea saponin by Aspergillus niger. Using the degradation rate of tea saponin, the fermentation time, temperature, initial pH of the culture medium, rotation speed and inoculum size were determined through the single factor experiment. The fermentation conditions were further optimized through the response surface design experiment. As results, the factors affecting the degradation rate were as follows： fermentation temperature〉pH of the culture medium〉 inoculum size〉fermentation time〉rotation speed. Finally, the optimum conditions for fermentation degradation were： fermentation time, 8 d; fermentation temperature, 38.7℃; initial pH of culture medium, 6.14; rotation speed, 125 r·min^-1 and inoculated size, 1.25%. Under such conditions, the degradation rate was 79.97%±0.82% that well matched with the theoretical value of 80.56%.

关 键 词：黑曲霉菌 液态发酵 茶皂素 条件优化 

分 类 号：S816.6[农业科学—饲料科学]