Bioinformatics Analysis of PHYB Gene in Upland Cotton(Gossypium hirsutum)  

陆地棉PHYB基因生物信息学分析（英文）

作　　者：沙琴[1] 杨建红 巩元勇[1] 郭书巧[1] 束红梅[1] 蒋璐[1] 倪万潮[1] 

机构地区：[1]江苏省农业科学院,农业部长江下游棉花和油菜重点实验室,江苏南京210014 [2]无棣县农业局,山东滨州251900

出　　处：《Agricultural Science & Technology》2016年第10期2256-2261,2353,共7页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31301682);the Agricultural Science Independent Innovation Foundation of Jiangsu Province[CX(14)5009];National Key Special Project for Breeding and Cultivation of GMO Varieties of China(2013ZX08005);the Jiangsu Province Science and Technology Support Program(BE2014389)~~

摘　　要：[Objective] This study was conducted to clarify the biological information of PHYB genes in upland cotton （Gossypium hirsutum）. [Method] Two PHYB genes were identified from the genome database of allotetraploid cotton （G. hirsutum L. acc. TM-1）, and were found to be distributed on subgenomes A10 and D10. And then bioinformatic analysis on these two genes were performed. [Result] The PHYB genes of upland cotton had the same motifs and domains with the PHYB genes in other plant species, and even the number and location of the motifs and domains of these PHYB genes were consistent. The PHYB amino acid sequence alignment and the phylogenetic tree constructed based on PHYB amino acid sequence of these plant species indicated that the two PHYB genes in upland cotton had higher homology and closer evolutionary relationships with cocoa （Theobroma cacao）, but lower similarity to PHYB genes in monocotyledonous plants, such as rice （Oryza saitva） and corn （Zea mays）. The comparison of PHYB gene structure also revealed that plant PHYB gene was more conserved during evolution. The autophosphorylation of dozens of phosphorylation sites in upland cotton PHYB gene may be essential for the functions of phytochromes and plays a significant role in regulating phytochrome-mediated signal transduction pathways. [Conclusion] The results of this paper will provide a theoretical basis for the cloning and functional research of PHYB genes.[目的]对陆地棉PHYB基因有一个全面的认识和生物信息学分析。[方法]在陆地棉异源四倍体标准系TM-1基因组数据库中共鉴定获得2个PHYB基因,这2个基因分别分布在A10和D10亚基因组。利用生物信息学方法对这两个基因进行初步分析。[结果]棉花的PHYB具有其他植物PHYB相同的基序和结构域,不管是数目还是分部位置都比较一致;不同植物的PHYB氨基酸序列同源性比对及构建的进化树都显示棉花的PHYB同可可的在同源性和进化关系上都是最高的,同水稻、玉米等单子叶植物的同源性及进化关系较低;基因结构的比对结果同样显示植物PHYB基因在进化上比较保守;棉花PHYB存在几十个可能磷酸化的位点,这些位点的磷酸化作用可能影响光敏色素的功能及其介导的信号通路。[结论]该研究结果为陆地棉PHYB基因的克隆和功能研究提供了理论基础。

关 键 词：Upland cotton （Gossypium hirsutum） Genome sequence of upland cotton PHYB gene BIOINFORMATICS 

分 类 号：S562[农业科学—作物学]