苜蓿菌根对土壤中阿特拉津降解及酶活性影响  

Effect of Alfalfa Mycorrhiza on Atrazine Degradation and Enzyme Activities in Soil

作　　者：宋福强[1] 范晓旭[1] 常伟[1] 李季泽[1] 吴奇[1] 周子鑫[1] 贾婷婷[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《中国农学通报》2016年第30期182-187,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金面上项目"丛枝菌根对阿特拉津污染土壤修复机制研究"(31270535);国家自然科学基金青年基金"阿特拉津胁迫下丛枝菌根与根际微生物互馈机制"(31500431);黑龙江省自然科学基金重点项目"菌根生物技术修复阿特拉津污染土壤作用机制研究"(ZD201206)

摘　　要：为进一步揭示丛枝菌根(AM)对土壤中阿特拉津的降解机理,建立了新型T型培养体系,并以阿特拉津敏感植物紫花苜蓿(Medicago sativa)接种摩西球囊霉(Glomus mosseae)形成的共生体系为研究对象,分别测定了苜蓿菌根对阿特拉津的降解效率及其根际土壤酶活性。当阿特拉津胁迫浓度为20 mg/kg时,苜蓿菌根侵染率在25天时高达85.70%,阿特拉津降解率为70.22%,其中菌根贡献度为77.28%,表明苜蓿菌根对阿特拉津具有较强的降解能力。与此同时,添加阿特拉津能够提高菌根根际土壤中蔗糖酶、脲酶、过氧化氢酶和纤维素酶的活性。在接种G.mosseae和添加阿特拉津的双重作用下,4种土壤酶活性进一步增强。总之,本研究结果为应用AM真菌修复农药污染土壤提供了理论依据。In order to reveal the degradation mechanism of AM on atrazine, the new cross- compartments cultivation systems（T type） were built. Medicago sativa which was sensitivity to atrazine was chosen to informsymbiotic association with Glomus mosseae as the research objective. Then the degradation rate of atrazine andsoil enzymes activities in rhizosphere were investigated in this study. When atrazine addition level was20 mg/kg, M. sativa root colonization reached 85.70% in 25 d, and the degradation rate of atrazine was70.22%, which was attributed to arbuscular mycorrhiza by 77.28%. It was suggested that alfalfa mycorrhizahad high degradation ability on atrazine in soil. Meanwhile, atrazine could promote the activities of saccharase,urease, catalase and cellulase in rhizosphere soil. Under the dual function of G. mosseae and atrazinetreatments, these four enzymes activities were further enhanced. In conclusion, this research provided theoretical foundation for AM application to remedy pesticide polluted soil.

关 键 词：阿特拉津 AM真菌 蔗糖酶 脲酶 过氧化氢酶 纤维素酶 

分 类 号：Q9[生物学]