地诺单抗对骨巨细胞瘤细胞功能的影响及其免疫效应的初步研究  

Immunological effects of denosumab on giant cell tumor of bone

作　　者：苏郁[1] 朱建福[1] 李婷[1,2] 王旭[1] 刘寿坤[1] 陈继良[1] 王体惠[1] 

机构地区：[1]福建医科大学附属闽东医院骨科分院,福安355000 [2]福建医科大学附属闽东医院病理科,福安355000

出　　处：《免疫学杂志》2016年第11期977-980,共4页Immunological Journal

基　　金：福建省自然科学基金(2016J01658)

摘　　要：目的观察地诺单抗对骨巨细胞瘤基质细胞的治疗作用,并探讨其在骨分化及免疫方面的机制,为地诺单抗的临床治疗应用提供理论依据。方法取临床肿瘤组织标本,体外培养获取原代骨巨细胞瘤基质细胞,加入地诺单抗,观察药物对基质细胞增殖与凋亡的影响,并进行成骨分化及相关免疫机制的探讨。结果当地诺单抗药物质量浓度为60、600及1 200μg/ml时,可显著抑制细胞增殖(P<0.05),并且药物质量浓度为600μg/ml时,抑制作用达到最大;与对照组相比,实验组的基质细胞的凋亡率显著升高(P<0.05);实验组的基质细胞的cbfa-1、osterix表达均显著升高(P<0.05);实验组的基质细胞分泌的IL-12及TNF-α明显升高(P<0.05)。结论与对照组相比,地诺单抗可显著抑制骨巨细胞瘤基质细胞的增殖,诱导凋亡,促进向成骨细胞分化,并增强机体的抗肿瘤免疫。To investigate the therapeutical effect of denosumab on giant cell tumor of bone,and discuss the mechanisms of osteoplastic differentiation and immune,the primary stroma cell was gained from the tumor tissue and cultured in vitro.After the stroma cells were adhered,the culture of experiment group was added with denosumab.Then,we observed the cell growth and apoptosis,and explored the mechanisms of osteoplastic differentiation and related immunological response.After treatment with denosumab of 60,600,and 1 200 μg/ml,the cell growth was obviously inhibited（P〈0.05）,and when the concentration was 600μg/ml,the inhibition reached the maximum.Thus,the optimal condition was the concentration of 600μg/ml.At that condition,the rate of apoptosis,the expression of cbfa-1 and osterix,and the secretion of IL-12 and TNF-α were all significantly higher than those of control group（P〈0.05）.Taken together,denosumab can inhibit the growth of stroma cell,induce the apoptosis,promote the osteoplastic differentiation,and enhance the antitumor immunity.

关 键 词：骨巨细胞瘤 基质细胞 地诺单抗 成骨诱导 抗肿瘤免疫 

分 类 号：R738.1[医药卫生—肿瘤]