纳米压痕技术测试Er:YAG激光辐射后牙本质的力学性能  

Mechanical properties of dentin after Er:YAG laser irradiation measured by nanoindentation

作　　者：何正娣 周莉[2] 大规昌兴 田上顺次[3] 陈玲玲[1] 阮双琛[1] HE Zheng-di ZHOU Li Masayuki Otsuki Junji Tagami CHEN Ling-ling RUAN Shuang-chen(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China)

机构地区：[1]深圳大学光电工程学院光电子器件与系统重点实验室,广东深圳518060 [2]深圳大学化学与化工学院,广东深圳518060 [3]日本东京医科齿科大学大学院齿学部龋病治疗学研究室

出　　处：《牙体牙髓牙周病学杂志》2016年第10期602-606,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(61505112);深圳市战略新兴产业发展专项资金(JCYJ20130402151227187)

摘　　要：目的：运用纳米压痕测量仪检测评估Er：YAG激光辐射对牙本质的纳米硬度和杨氏模量的影响。方法：选择新鲜拔除的健康人离体第三磨牙,制备成冠中部牙本质切片15个。将样本置于单脉冲（80 m J/脉冲）、水冷却模式的Er：YAG激光下辐射后,5个样本用于扫描电镜（SEM）观察激光辐射牙本质表面形态;其余10个样本沿辐射点的中心纵向切开,5个样本用于SEM观察激光辐射影响层内牙本质形态,另外5个样本用纳米压痕技术检测激光辐射影响层牙本质的纳米硬度及杨氏模量。结果：经Er：YAG激光辐射后,牙本质表面粗糙、呈鱼鳞状改变。辐射影响深度约10-15μm,激光辐射影响变质层牙本质的纳米硬度和杨氏模量均明显低于正常牙本质。结论：Er：YAG激光辐射可引起变质层牙本质的形态学及纳米硬度与杨氏模量的改变。AIM： To evaluate the nanohardness and Young＇ s modulus of dentin after Er： YAG laser irradia- tion using a nano -indentation tester. METHODS： 15 dentin disks obtained from extracted human third molars were prepared and exposed to a single pulse laser irradiation at 80 mJ/pulse. 5 specimens were observed for the morphology of laser - ablated dentin surface using a SEM. The remaining 10 specimens were crosscut through the expose center of the lased point. Among the 10, 5 were observed under SEM for the subsurface-lased dentin, 5 lased and 5 sound den- tin specimens were measured for nanohardness and Young＇ s modulus. RESULTS： The irradiated dentin surface was irregular, scaly or flaky. Laser irradiation denatured subsurface layer about 15 μm deep was present. The nanohardness and Young＇ s modulus of the Er： YAG laser denatured dentin were significantly lower than those of sound dentin （P 〈0.05）. CONCLUSION： Er：YAG laser irradiation can affect the nanohardness and Young＇ s modulus of dentin subsurface.

关 键 词：ER:YAG激光 牙本质 纳米压痕技术 纳米硬度 杨氏模量 

分 类 号：R781.05[医药卫生—口腔医学]