姜黄素抑制人神经胶质瘤U251细胞增殖的机制  

Mechanism of Curcumin in Inhibition of Proliferation of Human Glioma U251 Cells

作　　者：杨堃[1] 邓武生 李争争[1] 张家润[1] 郑传宜[1] 

机构地区：[1]海南医学院附属医院神经外科,海口570102

出　　处：《解放军医药杂志》2016年第10期43-45,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金(81260371)

摘　　要：目的探讨姜黄素对人神经胶质瘤U251细胞增殖的抑制作用及其相关机制。方法应用0、10、20、40、60、80μmol/L的姜黄素处理人神经胶质瘤U251细胞,检测细胞增殖、细胞周期、细胞凋亡及葡萄糖转运蛋白-3(GLUT-3)表达情况。结果 10、20、40、60、80μmol/L姜黄素作用于U251细胞吸光度(OD)值低于0μmol/L组(P<0.05)。姜黄素组作用72 h时人神经胶质瘤U251细胞G1期细胞比例少于对照组,G2/M期细胞比例多于对照组(P<0.05)。两组细胞凋亡率比较差异无统计学意义(P>0.05)。姜黄素组GLUT-3表达水平低于对照组(P<0.05)。结论姜黄素可以抑制人神经胶质瘤U251细胞增殖,其作用可能是通过下调GLUT-3的表达水平来实现的。Objective To investigate effect and mechanism of Curcumin in inhibition of proliferation of human glioma U251 cells. Methods Human glioma U251 cells were treated with 0,10,20,40,60 and 80 μmol / L Curcumin,and then cell proliferation and cycle,apoptosis,and glucose transporter type-3（ GLUT-3） protein expression were detected. Results Optical density（ OD） values of U251 cell after treatment with 10,20,40,60 and 80 μmol / L Curcumin were lower than that by 0 μmol / L（ P〈0. 05）. Cell radio of human glioma U251 cells in G1 phase after Curcumin treatment for 72 h was less,and the radio in G2 / M phase was more than those in control group（ P〈0. 05）. There was no significant difference in apoptosis rate between two groups（ P〉0. 05）. GLUT-3 expression in Curcumin group was lower than that in control group（ P〈0. 05）. Conclusion Curcumin can inhibit proliferation of human glioma U251 cells,and its mechanism may be achieved by down-regulated GLUT-3 expression.

关 键 词：姜黄素 神经胶质瘤 U251细胞 葡萄糖转运体-3 细胞增殖 

分 类 号：R730.264[医药卫生—肿瘤]