食品中胭脂虫红的高效液相色谱测定法  

Determination of carminic acid in food by HPLC

作　　者：郭蒙京[1] 李堃[1] 赵丹莹[1] 

机构地区：[1]北京市西城区疾病预防控制中心理化检验科,北京100120

出　　处：《职业与健康》2016年第18期2508-2510,共3页Occupation and Health

摘　　要：目的建立高效液相色谱法测定食品中胭脂虫红的方法。方法盐酸-甲醇提取,高效液相色谱-二极管阵列检测,保留时间定性,外标法定量。结果在4.0～100 mg/L时有良好线性关系,线性相关系数〉0.999,方法检出限为0.0004 g/kg,方法定量限为0.001 g/kg,加标回收率为92.3%～105.6%,相对标准偏差为0.7%～6.5%。结论该方法前处理简单实用,适用于各类食品中胭脂虫红的检测。[Objective]To establish a method for the determination of carminic acid in food by HPLC.[Methods]Carminic acid in food was extracted by hydrochloric acid-methanol and detected by HPLC with diode array detector. The qualitative and quantitative analysis was performed by retention time and external standard method,respectively.[Results]There was a good linear relationship in the concentrations of 4.0-100 mg/L,and the linear correlation coefficient was〉 0.999 9. The detection limit was 0.000 4 g/kg and quantitative limit was 0.001 g/kg. The recovery of standard addition was 92.3%-105.6%. The relative standard deviation was 0.7%-6.5%.[Conclusion]The pretreatment of the method is simple and practical,and this method is suitable for the determination of carminic acid in various kinds of food.

关 键 词：胭脂虫红 胭脂红酸 食品 高效液相色谱法 

分 类 号：R115[医药卫生—公共卫生与预防医学]