bFGF和MRP-1在胃癌中的表达及意义  

Expression and significance of bF GF and MRP-1 in gastric cancer

作　　者：谢家泰[1] 曾志荣[2] 

机构地区：[1]福建医科大学附属三明第一医院消化内科,365000 [2]中山大学附属第一医院消化科,510700

出　　处：《中国老年保健医学》2016年第5期13-15,共3页Chinese Journal of Geriatric Care

摘　　要：目的研究胃癌(GC)组织中碱性成纤维细胞生长因子(b FGF)和多重耐药蛋白1(MRP-1)的表达规律及其相互关系。方法用免疫组织化学染色(SP法)检测慢性浅表性胃炎(CSG)25例、胃黏膜肠上皮化生(IM)45例、异型增生(Dys)50例和GC 65例组织标本中b FGF、MRP-1阳性表达情况。RT-PCR技术检测MRP-1mRNA的表达情况。均数的比较采用t检验,两样本率的比较用χ2检验,两因素的相关分析用直线相关分析。结果在CSG→IM→Dys→GC的过程中,b FGF,MRP-1的表达呈逐步递增趋势,b FGF,MRP-1的阳性表达率GC组与Dys组、IM组,差异均具有统计学意义(P<0.05)。MRP-1与MRP-1mRNA表达强度呈正相关(r=0.598,P<0.05)。结论胃癌形成过程中,b FGF,MRP-1,MRP-1mRNA表达逐渐上调。Objectives To study the expression of basic fibroblast growth factor（ b FGF） and multidrug resistance protein 1（ MRP-1） in gastric cancer（ GC） tissue and their relationship. Methods immunohistochemical staining（ SP method） detecting the patients on chronic superficial gastritis（ CSG） 25 cases,Intestinal metaplasia（ IM） 45 cases,50 cases of dysplasia（ dys） and positive expression of 65 cases GC tissue specimens in b FGF and MRP-1. RT-PCR technology to detect the expression of MRP-1mRNA. The mean compared with t-test,the sample was compared with χ2-test,linear correlation analysis with correlation analysis of two factors.Results In the process of CSG,IM,Dys,GC,b FGF,MRP-1 expression was gradually increasing,bFGF,MRP-1 positive expression rate of GC group to Dys,IM group,the difference was statistically significant（ P〈0. 05）. MRP-1 expression intensity was positively correlated with MRP-1mRNA（ r = 0. 598,P〈0. 05）. Conclusions b FGF,MRP-1,MRP-1mRNA expression was gradually increased during the formation of gastric cancer.

关 键 词：胃癌 免疫组织化学染色 碱性成纤维细胞生长因子 多耐药相关蛋白1 逆转录-聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]