阿尔茨海默病和老年糖尿病患者的尿组蛋白变化及共病机制  

The level of histone in urine of patients with Alzheimer's disease or elderly patients with type 2 diabetes mellitus

作　　者：周静波[1] 梅少平[2] 王勇[3] 倪嘉缵[3] 宋达琳[1,4] 

机构地区：[1]青岛大学,266071 [2]青岛大学附属医院,266071 [3]深圳大学生命科学学院,518000 [4]青岛市市立医院,266071

出　　处：《中国老年保健医学》2016年第5期43-45,共3页Chinese Journal of Geriatric Care

基　　金：青岛市科技支撑项目12-1-4-16-(1)-jch

摘　　要：目的探讨阿尔茨海默病与老年2型糖尿病患者尿液中组蛋白的变化及其机制。方法对22例正常人、20例T2DM患者和20例AD患者的尿液标本进行多肽的富集,然后采用多肽组学分析技术进行检测并使用Protein Pilot软件(v4.5)对获得的数据进行检索比对分析,从而确定相应蛋白质的种类。结果与正常对照组相比AD组与T2DM组尿液中组蛋白H2B、组蛋白H2A、组蛋白H4显著增加。结论炎症机制可能导致AD与T2DM患者尿液中组蛋白含量增加。Objectives By investigate the level of histone in urine of patients with Alzheimer＇s disease（ AD） or elderly patients with type 2 diabetes mellitus（ T2DM） to seek whether there has common pathogenesis between AD and T2 DM. Methods The urine samples obtained from 22 healthy individuals and 20 patients with T2 DM and 20 patients with AD. The protein were analyzed by mass spectrometry after enrichment using weak cationic-exchange magnetic beads. Then the datum were analyzed by protein Pilot（ V. 4. 5） to determine the type of the protein. Results Compared with normal control group,the significant increased protein are histone H2 B,histone H2 A and histone H4 in both of AD and T2 DM patients. Conclusions Histone deacetylases and inflammation may have an effect on the high level of histone in urine.

关 键 词：阿尔茨海默病 老年2型糖尿病 尿液 多肽谱 组蛋白 

分 类 号：R749.16[医药卫生—神经病学与精神病学]