防己诺林碱抑制黑色素瘤的增殖转移  

Fangchinoline inhibits the growth and migration of melanoma

作　　者：郭冰玉[1] 张宇[1] 回蔷[1] 全亮亮[1] 陶凯[1] 

机构地区：[1]沈阳军区总医院整形外科,沈阳110840

出　　处：《实用药物与临床》2016年第10期1219-1223,共5页Practical Pharmacy and Clinical Remedies

摘　　要：目的观察防己诺林碱对黑色素瘤细胞生长转移的影响。方法通过MTT实验,观察不同浓度防己诺林碱对A375细胞增殖的影响;利用Transwell实验,检测不同浓度防己诺林碱对A375细胞转移功能的影响。通过Western blot和RT-PCR,检测防己诺林碱对A375生长转移相关蛋白的影响。结果防己诺林碱可以抑制黑色素瘤细胞A375的增殖转移。10、20、40μM/L防己诺林碱处理A375细胞后,对其增殖的抑制率分别为43.81%±1.53%、48.64%±4.65%、50.69%±4.99%。20、40μM/L防己诺林碱处理A375细胞作用24 h后,对其转移的抑制率分别为14.95%±4.31%、33.03%±5.46%。防己诺林碱显著抑制细胞周期蛋白D1(Cyclin D1)、CDK4、CDK6、基质金属蛋白酶-2(MMP2)的表达(P<0.05)。结论防己诺林碱能显著抑制A375细胞的增殖转移,可作为治疗黑色素瘤的潜在化疗药物进行深入研究。Objective To observe the effect of fangchinoline on growth and metastasis of melanoma cells.Methods MTT assay was used to observe the effect of different concentrations of fangchinoline on the proliferation of A375 cells.Transwell assay was used to observe the transfer function of A375 cells.Western blot and RT-PCR were used to detect the related proteins.Results Fangchinoline can inhibit the proliferation and migration of A375 cells.After being treated by 10,20 and 40μM /L of fangchinoline,the inhibition rates of proliferation of A375 cells were43.81%± 1.53%48.64%±4.65%and 50.69%±4.99%.After being treated by 20 and 40 μM /L of fangchinoline for 24 h,the inhibition rates of migration of A375 cells were 14.95%±4.31%and 33.03%± 5.46%.Fangchinoline could significantly inhibit the expression of Cyclin D1,CDK4,CDK6 and MMP2.Conclusion As a potential chemotherapeutic agent for treatment of melanoma,fangchinoline can significantly inhibit the proliferation and migration of A375 cells.

关 键 词：防己诺林碱 增殖 转移 A375 黑色素瘤 

分 类 号：R739.5[医药卫生—肿瘤]