非小细胞肺癌低表达miR-100与不良预后相关的临床研究  

Low expression of miR-100 and its poor prognosis related clinical research in non-small cell lung cancer

作　　者：杨丰名 管丹[1] 章海燕[2] 

机构地区：[1]南京医科大学第一附属医院肿瘤科,江苏南京210029 [2]沭阳县人民医院肿瘤血液科,江苏宿迁223600

出　　处：《中国肿瘤外科杂志》2016年第5期301-304,共4页Chinese Journal of Surgical Oncology

基　　金：国家自然科学基金(81272532)

摘　　要：目的研究microRNA-100(miR-100)在非小细胞肺癌中的表达水平及其与不良预后的相关性。方法从46例非小细胞肺癌组织及其相应癌旁组织中提取总RNA,使用qRT-PCR技术检测miR-100的表达量,分析miR-100表达水平与非小细胞肺癌预后之间的关系。结果 miR-100在40/46(86.9%)的肺癌肿瘤组织的表达要低于邻近正常组织;miR-100在Ⅱ期和Ⅲ、Ⅳ期患者的肿瘤组织中的相对表达水平要显著低于Ⅰ期患者;miR-100在化疗耐药的患者肿瘤组织中的相对表达水平也明显低于对化疗敏感的患者;miR-100低表达患者的DFS明显短于miR-100高表达患者。结论非小细胞肺癌癌组织中miR-100相对低表达与患者的不良预后相关,miR-100在未来有望作为预测肺癌预后的分子标志物之一。Objective To assess the expression level of microRNA-100 （miR-100） and its poor prognosis related clinical research in non-small cell lung cancer（NSCLC）. Methods Total RNA was extracted from 44 surgical specimens of NSCLC and corresponding adjacent tissues. MiR-100 expression was detected by qRT- PCR. The relationship between miR-100 expression level and prognosis was analyzed. Results MiR-100 expression was down-regulated in 86. 9% （40/46） of tumor tissue specimens. The relative expression level of miR- 100 in tumor tissues of patients with stage Ⅱ , Ⅲ and IV was significantly lower than that in patients with stage I. The relative expression level of miR-100 in patients with chemotherapy resistant patients was significantly lower than patients who respond to chemotherapy. Lower expression level of miR-100 was correlated with shorter dis- ease-free survival（DFS）. Conclusions The relative low expression of miR-100 is associated with poor progno- sis, and may be used as one of the molecular markers to predict the prognosis of lung cancer in the future.

关 键 词：非小细胞肺癌 miR-100 肿瘤标志物 预后 

分 类 号：R734.2[医药卫生—肿瘤]