N-cadherin在急性白血病患者骨髓白血病细胞中的表达及意义  

Expression and Clinical Significance of N-cadherin in Bone Marrow Leukemic Cells Derived from Patients with Acute Leukemia

作　　者：马杰[1] 刘亚杰[1] 张秋堂[1] 余庆峰[1] 张占芳[1] 张梦颖[1] 王冲[1] 甘思林[1] 陈胜梅[1] 曹伟杰[1] 谢新生[1] 刘延方[1] 刘林湘[1] 万鼎铭[1] 王芳[1] 孙玲[1] 孙慧[1] 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052

出　　处：《中国实验血液学杂志》2016年第5期1312-1318,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金(81000224);国家自然科学基金(81300418);河南省教育厅科学技术研究重点项目(13A320881)

摘　　要：目的:探讨神经钙粘蛋白(N-cadherin)在急性白血病患者骨髓白血病细胞中的表达及意义。方法:应用流式细胞术检测N-cadherin在113例急性白血病患者骨髓白血病细胞中的表达并分析其与临床特征及预后的相关性。结果:N-cadherin在急性白血病患者骨髓白血病细胞中的表达存在差异,表达水平波动在0%-99.7%;成人急性髓系白血病患者N-cadherin^+组CD34^+率较N-cadherin^-组显著升高(67.39%vs 33.33%)(P=0.013),两组间1疗程CR率、总CR率及复发率间的差异无统计学意义(P>0.05);急性淋巴细胞白血病患者N-cadherin^+组白细胞数(21.31±7.07 vs 51.10±23.69)(P=0.008)和外周血原始细胞比例〔(43.22±5.75)%vs(66.45±5.65)%(P=0.015)〕显著低于N-cadherin^-组,1疗程CR率和总CR率低于N-cadherin-组,复发率高于N-cadherin-组,但差异无统计学意义(P>0.05);儿童急性淋巴细胞白血病患者N-cadherin^+组CD33^+率较N-cadherin-组显著增高(47.62%vs 0%)(P=0.012),两组间1疗程CR率和总CR率无显著差异。N-cadherin^+组复发率高于N-cadherin^-组(30.00%vs 0%)(P=0.115)。成人AML、非M3-AML、ALL和儿童ALL患者N-cadherin^-组中位生存时间、3年总生存(OS)率和3年无复发生存率均好于N-cadherin^+组,但其差异无统计学意义。结论:N-cadherin在急性白血病患者骨髓白血病细胞中的表达水平与部分临床特征有关,对急性白血病患者的生存有一定影响。Objective：To investigate the expression of N-eadherin in bone marrow leukemic cells derived from acute leukemia patients and its clinical significances.Methods：A total of 113 patients with acute leukemia were enrolled in this study.Flow cytometry was employed to detect the expression of N-Cadherin in bone marrow leukemic cells from acute leukemia patients and the relationships between the N-eadherin expression and the clinical characteristics of patients with acute leukemia were analyzed.Results：The expression of N-Cadherin in bone marrow leukemic cells deriveted from patients with acute leukemia was variable with 0%-99.7%.For adult AML patients,the positive rate of CD34 in N-cadherin＋ group was significantly higher than that in N-eadherin group（67.39%vs 33.33%）（P=0.013）,while the differences of total CR rate and rate of CR after 1 cycle of induction treatment were not significant between these 2groups（P 〉0.05）.As to ALL patients,N-cadherin＋ group had significant lower WBC count（21.31±7.07 vs 51.10±23.69）（P= 0.008） and lower percentage of peripheral blood blast（43.22±5.75%vs 66.45±5.65%）（P=0.015）.The CR rate after 1 cycle of induction treatment and rate of overall CR were lower and the relapse rate was higher in N-cadherin＋ ALL group than those in N-cadherin ALL group,but the differences were not significant（P〉0.05）.For childhood ALL,the positive rate of CD33 in N-cadherin＋ group was significantly higher than that in Ncadherin group（47.62%vs 0%）（P= 0.012）.The relapse rate was higher in N-cadherin＋ group than that in Ncadherin group（30.00%vs 0%）（P=0.115）.The median survival time,3-year overall OS rate and 3-year relapsefree survival rate in N-cadherin groups of adult AML,non-M3 AML,ALL and chidhood ALL paients were superior to N-cadherin＋ groups,but the differences were not significant.Conclusion：The expression of N-cadherin in bone marrow leukemic cells relates to some clinical features of patients with acute leukemia and to some extent h

关 键 词：急性白血病 骨髓白血病细胞 N-CADHERIN 临床意义 

分 类 号：R733.71[医药卫生—肿瘤]