急性髓系白血病患者胰岛素样生长因子结合蛋白3的表达及其意义  

Expression and Significance of Insulin-like Growth Factor Binding Protein 3 in Patients with Acute Myeloid Leukemia

作　　者：孙洪芝[1] 孟凡杰[1] 郭恒岳[2] 

机构地区：[1]潍坊市益都中心医院检验科,山东青州262500 [2]潍坊市益都中心医院血液科,山东青州262500

出　　处：《中国实验血液学杂志》2016年第5期1319-1323,共5页Journal of Experimental Hematology

摘　　要：目的:探讨急性髓系白血病患者骨髓单个核细胞中胰岛素样生长因子结合蛋白3(IGFBP3)的基因表达和外周血中的IGFBP3水平及其临床意义。方法:将2014年3月至2016年3月期间178例AML病人按照病情分为初治组,缓解组和复发组;并选取同时期的骨髓象正常的非恶性血液病患者作为对照组,利用ELISA检测各组外周血中的IGFBP3水平,利用实时荧光定量PCR方法检测骨髓单个核细胞中IGFBP3的基因表达水平。结果:IGFBP3基因在初治组和复发组病人骨髓单个核细胞中的表达水平明显的低于对照组(P<0.05),而缓解组与对照组,初治组与复发组之间无显著的差异(P>0.05);IGFBP3蛋白在初治组和复发组病人外周血中的水平明显的低于对照组(P<0.05),而缓解组与对照组,初治组与复发组之间无显著的差异(P>0.05);IGFBP3在外周血和骨髓单个核细胞中的表达水平无显著的相关性。结论:AML病人骨髓单个核细胞中的IGFBP3基因表达以及外周血中的IGFBP可能对AM L的发生、发展起到了一定的作用,其还可以在一定程度上对病情以及治疗效果做出评估。Objective：To explore the gene expression of insulin-like growth factor binding protein 3（IGFBP3） in bone marrow mononuclear cells and the expression of IGFBP3 in peripheral blood as well their significance.Methods：A total of 178 patients with acute myeloid leukemia（AML） from March 2014 to March 2016 were divided into de novo AML,CR,and relapse groups according to their condition;Patients with non-hematologic malignancies and normal bone marrow in the same period were selected as control group.The ELISA method was used to detect the IGFBP3 protein levels in peripheral blood,and PCR method were used to detecte IGFBP3 gene expression in bone marrow mononucleated cells.Results：IGFBP3 gene expression levels of bone marrow mononuclear cells in de novo AML and relapse groups were significantly lower than that in control group（P〈0.05）,while that in CR group and control group,de novo AML and relapse groups was no significantly different（P 〉 0.05）;IGFBP3 levels of peripheral blood in de novo AML and relapse groups were significantly lower than those in control group（P〈0.05）,while those in CR group and control group,de novo AML and relapse groups were no significantly different（P 〉 0.05）;IGFBP3 expression levels in peripheral blood and bone marrow mononucleated cells did not show significant correlation.Conclusion：The gene expression of IGFBP3 in bone marrow mononuclear cells and its protein levels in peripheral blood may play an important role in occurrence and development of acute myeloid leukemia,and it can also evaluate the disease status and treatment efficacy of acute myeloid leukemia in some extent.

关 键 词：急性髓系白血病 胰岛素样生长因子结合蛋白3基因表达 蛋白水平 

分 类 号：R733.71[医药卫生—肿瘤]