急性髂股静脉血栓模型大鼠血清D-二聚体、纤维蛋白原、C反应蛋白、白介素6与血栓溶解体积之间的关联研究  

Relationship of Serum D-dimer,Fibrinogen,C Reactive Protein and Interleukin 6 with Thrombus Dissolution Volume in Acute Iliac Femoral Vein Thrombosis Model Rats

作　　者：王琦[1] 孙思翘[1] 程志华[1] 王中英[1] 刘超[2] 

机构地区：[1]吉林大学第一医院血管外科,吉林长春130021 [2]天津市胸科医院心内科,天津300222

出　　处：《中国实验血液学杂志》2016年第5期1523-1528,共6页Journal of Experimental Hematology

基　　金：2015年中国博士后科学基金资助项目(2015M581308)

摘　　要：目的:探究急性髂股静脉血栓模型大鼠血清D-二聚体、纤维蛋白原、C反应蛋白、白介素6与血栓溶解体积之间的关联。方法:选择清洁级健康SD大鼠60只,制作急性髂股静脉血栓模型,按照处置方法的不同分为3组,即模型组、假手术组和正常对照组,每组20只大鼠。分别于造模前和造模成功后不同时间点进行D-二聚体、纤维蛋白原、C反应蛋白和白介素-6含量测定,并且计算静脉造影所显示的血栓溶解体积,对检测结果进行统计学分析。结果:大鼠模型组和假手术组在手术后血清D-二聚体和纤维蛋白原测量值均先升高后降低,模型组出现2次峰值,假手术组升高3 h达到峰值后即逐渐下降,至术后24 h明显降低(P<0.05)。模型组和假手术组在手术后血清C反应蛋白和白介素6含量均先升高后降低,且模型组含量明显高于假手术组。各时间点的D-二聚体测量值与相应的血栓溶解体积之间呈正相关的关系,Pearson系数为0.307,R2=0.412(P<0.05)。结论:急性髂股静脉血栓形成模型大鼠血清D-二聚体、纤维蛋白原、C反应蛋白、白介素6随着时间呈现均先升高后降低的变化规律,这些变化能够反映髂股静脉血栓形成过程中血液凝聚状态和纤维蛋白溶解过程。Objective：To explore the relationship among the serum D-two polymer,fibrinogen,C reactive protein and interleukin 6 and thrombus dissolution volume in acute iliac femoral venous thrombosis model rats.Methods：A total of 60 rats were randomly divided into 3 groups：deep venous thrombosis group（DVT group）,sham operation group and normal control group.In DVT group the single side of the iliofemoral vein incomplete with micro vessel was cliped under chloral hydrate anesthesia;in sham operation group the single side of the iliofemoral vein should be explored without using micro vessel clip under chloral hydrate anesthesia;the and normal control group only experienced chloral hydrate anesthesia.A positive correlation was showed between the 2 time points of D-dimer and the corresponding thrombolytic volume,and the Pearson coefficient was 0.307,and R was 0.412（P〈0.05）.Results：The D-dimer,fibrinogen,C reactive protein and interleukin-6 levels before and after treatment of 60 rats were shown to be significantly different（P〈0.05） between DVT group,sham operation group and normal control group.The D-dimer and fibrinogen level was first rised and then decreased in DVT group,sham operation group.There was a positive correlation between C reactive protein/interleukin-6 and the level of D-dimer /fibrinogen from T1 to T3 time point（P〈0.05）.There was a negative correlation between C reactive protein/interleukin-6 and the level of D-dimer /fibrinogen from T4 to T6 time point（P〈0.05）.Conclusion：The changes of serum D-dimer,fibrinogen,C reactive protein and interleukin 6 in the acute iliac femoral vein thrombosis model firstly increase and then decrease.These changes can reflect the process of blood coagulation and fibrin dissolution in the course of venous thrombosis of iliac vein.

关 键 词：D-二聚体 深静脉血栓 纤维蛋白原 C反应蛋白 白介素6 血栓溶解体积 关联研究 

分 类 号：R364.15[医药卫生—病理学]