miR-200b与癌胚抗原在大肠癌中的表达及其相关性  

The expression of miR-200b and CEA and the correlation between the two factors in colon cancer

作　　者：杜叶平[1] 王海莲[1] 尹莉莉[1] 武春梅[1] 苗晋华[1] 

机构地区：[1]中国人民解放军第二六四医院检验科,太原030001

出　　处：《国际检验医学杂志》2016年第20期2808-2809,2812,共3页International Journal of Laboratory Medicine

基　　金：山西省自然科学基金资助项目(2013011043-4)

摘　　要：目的探讨miR-200b与癌胚抗原(CEA)在大肠癌发生、发展中的表达情况及其表达的相关性。方法采用实时荧光定量PCR方法分析60例大肠癌组织及正常组织中miR-200bmRNA和CEA mRNA表达结果,分析其与患者临床资料、病理参数之间的关系,研究大肠癌组织中miR-200bmRNA和CEA mRNA表达的相关性。结果大肠癌组织中miR-200bmRNA的表达显著低于正常组织,CEA mRNA显著高于正常组织,差异均有统计学意义(P<0.01);患者性别、年龄及肿瘤部位的miR-200bmRNA和CEA mRNA表达结果比较,差异无统计学意义(P>0.05);不同组织学分级及TNM分期中,CEA呈现过表达状态,而miR-200b呈现表达降低或缺失状态;大肠癌组织中miR-200b和CEA的表达具有相关性,且呈负相关关系(r=-0.790,P<0.001)。结论 miR-200b在大肠癌组织中呈低表达或表达缺失,与CEA的表达呈一定的负相关关系,提示miR-200b可为肿瘤的诊断和治疗提供新的依据。Objective To study the expression of transcription factor miR-200 band CEA and the correlation between the two transcription factors in the process of occurrence and development of colorectal cancer.Methods Expression miR-200 band CEA mRNA by real-time PCR in 60 colon cancer tissues and corresponding normal tissues were detected and the results were compared with the clinical features and pathological characters.The relationship between the mRNA expression of miR-200 band CEA in 60 colon cancer tissues was determined.Results The expression rate of CEA mRNA was detectable to highly expressed rates in colon cancer tissues than that in matched normal tissues（P〈0.01）,whereas miR-200 bmRNA in the colon cancer tissues was significantly lower than that in the matched normal tissues（P〈0.01）.There was no significant correlation between miR-200 b,CEA mRNA expression and age,sex,tumor location（P〈0.05）.Loss expression or down regulation expression of miR-200 bmRNA was detected,whereas CEA mRNA was detectable to highly expressed in the different histological grade and Dukes stages.The expression of miR-200 bmRNA and CEA mRNA were positively correlated with the histological grade in colon cancer.A significant correlation was found between the expression of miR-200 bmRNA and CEA mRNA（r=-0.790,P〈0.001）.Conclusion Loss or down regulation expression of miR-200 bmRNA,whereas CEA is detectable to highly expressed in colon cancer.Negative correlation occurred in miR-200 bmRNA and CEA mRNA indicates that miR-200 band CEA provide the new clues of genetic diagnosis and treatment.

关 键 词：大肠癌 miR-200b 癌胚抗原 实时定量PCR 

分 类 号：R735.34[医药卫生—肿瘤]