卵巢上皮性癌组织中SP17、SPAG9、OY-TES-1基因的去甲基化表达及其与临床病理的关系  

Demethylation expressions of SP17,SPAG9 and OY-TES-1 in ovarian epithelial cancer tissues and their relationship with clinicopathological features

作　　者：崔梦瑶 岑尧 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010010 [2]内蒙古自治区人民医院妇科,内蒙古呼和浩特010010

出　　处：《中国临床研究》2016年第10期1307-1310,1314,共5页Chinese Journal of Clinical Research

基　　金：内蒙古自治区自然科学基金项目(2013MS1148)

摘　　要：目的检测在卵巢上皮性癌与非卵巢癌中,精子蛋白17（SP17）、精子相关抗原9（SPAG9）、OY-TES-1这三种癌-睾丸抗原基因启动子区域去甲基化表达的差异,探讨其与卵巢上皮性癌临床病理分期的关系。方法收集2015年1月至2016年4月25例新鲜的卵巢上皮性癌组织标本,及25例新鲜的非卵巢癌组织标本,采用DNA酸性亚硫酸盐转化、PCR扩增及甲基化敏感性内切酶方法检测SP17、SPAG9、OY-TES-1在卵巢上皮性癌与非卵巢癌中Cp G岛的去甲基化表达。结果与非卵巢癌组织比较,卵巢上皮性癌组织中SP17、SPAG9、OY-TES-1基因启动子区存在高去甲基化表达（P均〈0.01）。三种癌-睾丸抗原基因启动子区域去甲基化表达阳性率在卵巢上皮性癌不同年龄、组织学类型和分化程度中比较,差异无统计学意义（P均〉0.05）;SP17、SPAG9启动子区域去甲基化表达阳性率在有淋巴结转移者中高于无淋巴结转移者（P〈0.01,P〈0.05）,在Ⅲ-Ⅳ期患者中高于Ⅰ-Ⅱ期患者（P均〈0.05）;而OY-TES-1启动子区域去甲基化表达阳性率仅在有淋巴结转移者中高于无淋巴结转移者（P〈0.05）。结论 SP17、SPAG9、OY-TES-1基因启动子区域在卵巢上皮性癌中存在高去甲基化表达,且其去甲基化表达均与患者发病年龄、组织学类型、分化程度无关;部分与淋巴结转移、肿瘤分期相关。SP17、SPAG9、OY-TES-1或可成为卵巢上皮性癌免疫治疗的靶位点。Objective To detect demethylation expressions of cancer-testis antigens [sperm protein 17（ SP17）,sperm associated antigen 9（ SPAG9） and OY-TES-1] of gene promotor regions in ovarian epithelial cancer and non ovarian cancer tissues,and to analyze the association with demethylation expression and clinicopathological staging of ovarian epithelial cancer. Methods From Jannary 2015 to April 2016,twenty-five fresh samples of ovarian epithelial cancer and 25 fresh samples of non ovarian cancer tissues were collected. DNA acid bisulfite conversion,PCR amplification and methylation sensitive inner enzyme digestion method were used to detect the demethylation expressions of SP17,SPAG9 and OY-TES-1in Cp G islands of ovarian epithelial cancer and non ovarian cancer tissues. Results Compared with non ovarian cancer tissues,SP17,SPAG9 and OY-TES-1 in gene promotor regions presented high demethylation expressions in ovarian epithelial cancer tissues（ all P〈0. 01）. There were no significant differences in positive demethylation expression rates of these three kinds of cancer-testis antigen of gene promotor regions in age,histological types and degree of tumor differentiation（ all P〈0. 05）. The positive demethylation expression rates of SP17 and SPAG9 of promotor regions were significantly higher in patients with lymph node metastasis than those in patients without lymph node metastasis（ P〈0. 01,P〈0. 05）,and also significantly higher in patients with TNM stage Ⅲ-Ⅳ than those in patients with TNM stageⅠ-Ⅱ（ all P〈0. 05）,while the positive demethylation expression rate of OY-TES-1of promotor regions was higher in patients with lymph node metastasis than that in patients without lymph node metastasis only（ P〈0. 05）. Conclusions SP17,SPAG9 and OY-TES-1 of gene promotor regions present high demethylation expressions in ovarian epithelial cancer tissues. The demethylation expressions are associated with lymph node metastasis and tumor staging in some patients,but not with the age of onset,

关 键 词：癌-睾丸抗原 精子蛋白17 精子相关抗原9 OY-TES-1 上皮性卵巢癌 去甲基化 临床病理特征 

分 类 号：R737.31[医药卫生—肿瘤]