Th17及Treg相关因子在反复种植失败患者种植窗口期子宫内膜中的表达  

Expression of Th17 and Treg in endometrium during implantation window phase in patients with repeated implant failure

作　　者：艾海权[1] 腊晓琳[1] 巩晓芸[1] 李霞[1] 朱玥洁[1] 丁剑冰[2] 玛依热.吐尔逊 

机构地区：[1]新疆医科大学第一附属医院妇科生殖助孕中心生殖医学科,乌鲁木齐830054 [2]新疆医科大学基础医学院免疫教研室,乌鲁木齐830011

出　　处：《生殖医学杂志》2016年第11期1013-1017,共5页Journal of Reproductive Medicine

基　　金：新疆医科大学第一附属医院自然基金重点项目(2013ZRZD019)

摘　　要：目的通过研究种植窗口期子宫内膜中Treg及Th17特异性转录因子及相关细胞因子表达情况,探讨反复种植失败患者子宫内膜中Treg/Th17平衡调节的可能机制。方法建立种植窗口期子宫内膜标本库。从标本库选择2013年10月至2014年12月收集的符合实验要求的反复种植失败患者(实验组)及首次移植妊娠患者(对照组)各20例,采用定量RT-PCR测量内膜标本中的Treg、Th17特异性表达因子Foxp3和ROR-γt及相关细胞因子IL-10、IL-17表达情况。结果在实验组和对照组子宫内膜上均可见Foxp3、ROR-γt及细胞因子IL-10、IL-17表达;与对照组比较,实验组Foxp3mRNA的相对表达量[(0.77±0.15)vs.(1.57±1.22)]及IL-10mRNA的相对表达量[(1.14±1.03)vs.(1.54±1.77)]均显著降低(P<0.05),而ROR-γt mRNA的相对表达量[(0.92±0.39)vs.(0.55±0.15)]及IL-17mRNA的相对表达量[(0.95±0.31)vs.(0.49±0.21)]均显著升高(P<0.05)。结论 IL-17、IL-10可能参与内膜中Th17/Treg平衡调节。Objective： To investigate the expression of specific transcription factors and related cytokines of Treg and Th17 in the endometrium,and explore the mechanism of regulation balance of Th17/Treg in patients with repeated implant failure. Methods： The endometrial specimens bank in the implantation window phase was established. A total of 40 specimens met the experimental requirements were collected from October 2013 to December 2014. Among them, 20 specimens were from the patients with repeated implantation failure as experimental group ,and another 20 specimens from the pregnancy patients in their first transplantation as the control group. The expressions of Foxp3,ROR-γt,IL-10,and IL-17 in endometrial specimens were measured by quantitative RT-PCR.Results： Foxp3,ROR-）,t,IL-10, and IL-17 in endometrium were expressed in both experimental group and control group. ThemRNA expressions of Foxp3（0.77 ± 0.15 vs. 1.57 ± 1.22）and IL-10（1.14 ± 1.03 vs. 1.54 ± 1.77）in the experimental group were significantly lower than those in the control group （P〈0.05）. The mRNA expressions of ROR-γt（0.92 ± 0.39 vs. 0.55 ± 0.15）and IL-17（0.95 ± 0.31 vs. 0.49 ± 0.21） in the experimental group were significantly higher than those in the control group （P〈0.05）.Conclusions： IL-10 and IL-17 may involve in regulation balance of Th17/Treg.

关 键 词：TREG TH17 反复种植失败 子宫内膜容受性 种植窗 

分 类 号：R[医药卫生]