姜黄素联合紫杉醇对人口腔鳞癌细胞的增殖抑制及凋亡诱导作用  

Roles of curcumin combined with paclitaxel on growth inhibition and apoptosis of oral squamous cell carcinoma cell line CAL27 in vitro

作　　者：刘敏[1,2] 张君[1] 李俊福[2] 王旭霞[1,3] 

机构地区：[1]山东大学口腔医学院,山东济南250012 [2]泰山医学院附属医院,山东泰安271016 [3]山东省口腔生物医学重点实验室,山东济南250012

出　　处：《上海口腔医学》2016年第5期538-541,共4页Shanghai Journal of Stomatology

基　　金：山东省科学技术发展项目(2010GSF10239);山东省科学技术专项计划项目(2012G0021852)

摘　　要：目的:探讨姜黄素和紫杉醇对人口腔鳞癌细胞系CAL27的增殖和凋亡的影响。方法:培养人口腔鳞癌细胞系CAL27,采用不同浓度的姜黄素和紫杉醇及联合用药处理细胞24 h、48 h及72 h。MTT法检测对细胞增殖的影响,Tunel法检测对细胞凋亡的影响,Western印迹法检测对凋亡相关蛋白Bcl-2、Bax和活性caspase-3表达的影响。采用SPSS11.0软件包对数据进行统计学分析。结果:相对于姜黄素或紫杉醇对CAL27的单独作用,两者联合用药能更显著地抑制CAL27细胞增殖和促进CAL27细胞凋亡,更显著地下调Bcl-2表达和Bcl-2/Bax表达比例,上调Bax和活性caspase-3的表达。结论:相比于单一用药,姜黄素和紫杉醇联合用药对CAL27有更好的增殖抑制和凋亡诱导作用。PURPOSE： To investigate the effects of curcumin and paclitaxel on proliferation and apoptosis of human oral squamous carcinoma cell line CAL27. METHODS： CAL27 cells were cultured and treated with different concentrations of curcumin and paclitaxel separately or in combination for 24, 48 and 72 h. The cell proliferation was evaluated by MTY assay, and cell apoptosis was detected by Tunel staining. Expression of Bcl-2, Bax, and active caspase-3 were analyzed by Western blot. The data were analyzed with SPSS 11.0 software package. RESULTS： Compared with the effects of curcumin or paclitaxel, combined treatment of both drugs significantly inhibited cell growth, induced cell apoptosis, decreased the expression of Bcl-2 and the ratio of Bcl-2/Bax, and increased the expression of Bax and active caspase-3 in CAL 27 cells. CONCLUSIONS： Combined treatment of Curcumin and Paclitaxel significantly inhibits cell growth and mediated cell apoptosis compared with that of either single drug.

关 键 词：口腔鳞癌 姜黄素 紫杉醇 细胞凋亡 

分 类 号：R739.85[医药卫生—肿瘤]