不同来源芍药的质量分析  

Analysis of Quality of Chinese Herbaceous Peony of Different Sources

作　　者：张宏莲[1] 孙辑凯[1] 张梅娟[2] 董巍[1] 沙伟[2] 朱巍巍[2] 

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161000 [2]齐齐哈尔大学,黑龙江齐齐哈尔161000

出　　处：《中国卫生产业》2016年第31期42-44,共3页China Health Industry

基　　金：齐齐哈尔市科技局农业攻关项目<野生赤芍种子繁育及发育机理研究>;项目编号:NYGG-201404

摘　　要：目的通过实验,检视野生、市售及药用植物园栽培赤芍中所含成分的数目,测定指标成分芍药苷含量,作为评价栽培赤芍质量优劣的科学依据。方法采用薄层色谱法,将对照溶液及供试液点样10μL于GF254板上,以三氯甲烷-乙酸乙酯-甲醇-甲酸(40∶5∶10∶0.2)为展开剂,254 nm下检视;采用HPLC法,条件是采用Waters E2695色谱系统,Agilent ZORBAX Eclipse XDB-C18色谱柱(250×9.4 mm,5μm);以甲醇-0.05 mol/L磷酸二氢钾溶液(50∶50)为流动相,流速1.0 m L/min,检测波长230 nm,进样量10μL。结果在254 nm下检视,野生、市售及药用植物园栽培赤芍的TLC板明显的荧光斑个数分别为6、11、10;芍药苷的保留时间是5.078 min,野生、市售、栽培赤芍中芍药苷的含量分别为6.6%、7.4%、7.0%。结论依据TLC检视及HPLC结果可知,野生、市售及药用植物园栽培赤芍所含化学成分的种类有显著差异,不同来源的芍药中芍药苷的含量相近、相差不显著。Objective To measure the peoniflorin content of marker substances as a scientific basis of evaluating the cultivatedred paeonia quality degree by experiment and viewing the content number in the in the wild, commercially availableand cultivated red paeonia in the medicinal botanical garden. Methods The 10 滋L contrast solution and test solution sampleswere put on the GF254 plate by the thin-layer chromatography, and viewed under 254 nm with chloroform - ethyl acetate- methanol - formic acid （405：10：0.2） as the agent, HPLC method and Waters E2695 chromatographic system wereadopted, chromatography was performed on Agilent ZORBAX Eclipse XDB-C18 column （250x9.4 mm, 5 滋m）, withmethanol -0.05 mol/L potassium dihydrogen phosphate solution （50：50） as the mobile phase at a flow rate of 1.0 ml ？ min-1.The absorbance was monitored at 230 nm. The injection volume was 10 滋L. Results Under the view of 254 nm, the obviousfluorescent spot numbers on TLC plate in the wild, commercially available and the cultivated red paeonia in the medicinalbotanical garden were respectively 6, 11 and 10, and the retention time of paeoniflorin was 5.078 min, and the contentof paeoniflorin in the wild, commercially available and cultivated red paeonia was respectively 6.6%, 7.4% and 7.0%. ConclusionThe TLC view and HPLC results show that there are obvious differences in the chemical component type in thewild, commercially available and cultivated red paeonia in the medicinal botanical garden, and the content of paeoniflorin inthe Chinese herbaceous peony of different sources is similar and the difference is not obvious.

关 键 词：芍药 芍药苷 薄层色谱法 质量分析 

分 类 号：R914.1[医药卫生—药物化学]