表没食子儿茶素没食子酸酯对活性氧的抑制作用  

Inhibitory Effects of(-)-Epigallocatechin-3-gallate on Reactive Oxygen Species

作　　者：廖香莲[1] 林慧韫[1] 李步洪[1] 

机构地区：[1]福建师范大学医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,福建福州350007

出　　处：《激光生物学报》2016年第5期406-412,430,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61505031,61275216);国家卫计委科学研究基金(WJK-FJ-30);福建省自然科学基金项目(2014J07008,2011J01342)

摘　　要：生物体内的活性氧（Reactive oxygen species,ROS）过量引起氧化应激将导致脂质、DNA和蛋白质氧化损伤,从而引发一系列生理和病理反应。绿茶中茶多酚的主要成分表没食子儿茶素没食子酸酯（（-）-Epigallocatechin-3-gallate,EGCG）具有强抗氧化性,能有效抑制ROS。本文简要介绍了生物体内ROS的来源和EGCG的特性及其对ROS的抑制作用。通过检测玫瑰红水溶液在光敏化时所产生^1O2的1 270 nm近红外发光,分析比较了EGCG和迭代钠（NaN3）对^1O2发光的淬灭过程,发现EGCG对^1O2的淬灭效果比NaN3更好,为EGCG淬灭^1O2的定量研究提供理论依据。Reactive oxygen species（ ROS） overproduction could cause oxidative stress,leading to oxidative damage of lipids,DNA and protein,and also triggering a series of physiological and pathological reactions.（-）-Epigallocatechin-3-gallate（ EGCG） is the main component of polyphenols in green tea,which has a strong antioxidant activity and could effectively inhibit the generation of ROS. In this paper,the origination and physiological role of ROS,and the characteristics of EGCG and its inhibited effects on ROS will be introduced. In addition,the quenching effects of EGCG on singlet oxygen（^1O2） generation during photosensitization of Rose Bengal（ RB） in aqueous solution has been investigated by the direct detection of ^1O2 luminescence at 1 270 nm,the quench effects of EGCG and sodium azide（ NaN3） for ^1O2 was comparatively analyzed. It was concluded that the quenching effects of EGCG on ^1O2 was superior to NaN3,providing a theoretical basis for quantitative study of EGCG quenching effects on ^1O2.

关 键 词：表没食子儿茶素没食子酸酯 活性氧 抑制作用 ^1O2 淬灭 

分 类 号：R318.51[医药卫生—生物医学工程]