鞘内置管技术在大鼠胸髓损伤模型中的改进及应用  

Improvement and application of intrathecal catheterization in rat with thoracic cord injury

作　　者：李向哲[1] 谢财忠[2] 王娜[1] 宋新建[3] 吴勤峰[4] 方露[1] 王彤 

机构地区：[1]南京医科大学第一临床医学院,江苏南京210029 [2]南京军区南京总医院康复科,江苏南京210002 [3]南通市第二人民医院康复科,江苏南通226002 [4]南通大学附属医院康复中心,江苏南通226001 [5]南京医科大学第一附属医院康复中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2016年第10期1202-1207,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81171851)

摘　　要：目的 ：探索一种适用于T10脊髓损伤（spinal cord injury,SCI）大鼠模型的鞘内置管法,为SCI模型提供更佳的蛛网膜下腔给药方案。方法：选择60只雌性SD大鼠,随机分为L3～4置管组（T组）和L6～S1置管组（C组）。对比两组置管和SCI后致残率、死亡率、咬管率、利多卡因试验、体重变化和蓝墨水定位试验情况,并在SCI后1周行Alzet渗透压泵植入术,观察长期置管和渗透压泵植入对大鼠死亡率、体重和脊髓形态的影响。结果：置管及SCI后7 d,两组咬管率对比具有明显差异（P〈0.05）,总淘汰率具有显著差异（P〈0.01）;置管及SCI后两组大鼠体重均出现下降,置管后第35天T组大鼠体重高于C组（P〈0.05）;蓝墨水定位试验显示,两组导管均通畅且位置未出现明显偏移;Alzet渗透压泵植入4周,组织相容性良好;HE染色显示,两组腰髓后角均受到轻度压迫。结论：经L3～4置管法致残率和咬管率低,且更易操作,是一种较为理想的SCI模型鞘内置管法。Objective：To explore an intrathecal catheterization method that suitable for T10 spinal cord injury（SCI） rats model,which can be better used for dosing subarachnoid space in（spinal cord injury,SCI） model. Methods：A total of 60 female SD rats were randomly divided into the L3 ～4 catheterization group（T group） and the L6 ～S1 catheterization group（C group）. After 7 days of intrathecal catheterization and SCI,the differences between two types of intrathecal catheterization in disability rate,mortality,biting catheter rate,lidocaine test,weight changes and blue ink location test were compared. After 7 days of SCI,Alzet osmotic pumps were implanted into two groups respectively,then we observed the mortality,weight and the morphology of the spinal cord after long-term catheter and osmotic pumps implanted in SCI rats. Results：After 7 days of intrathecal catheterization and SCI,the biting catheter rate of the two groups had significant difference（P〈0.05）,and total elimination rate had significant difference（P〈0.01）. After intrathecal catheterization and SCI,the weights of the two groups were decreased,and after 35 days of intrathecal catheterization,the weight of rats in the T group was higher than that in the C group（P〈0.05）. Blue ink location test showed that there was no appearance of catheter obstruction and position migration in the two groups. After 4 weeks of Alzet osmotic pumps implanted,the histocompatibility of Alzet osmotic pumps were well. HE staining showed that the lumbar spinal cords in the two groups were all been compressed mildly. Conclusion：The L3 ～4 intrathecal catheterization is ideal for SCI model in rats as the biting catheter rate and mortality is lower,and the operation is easier.

关 键 词：大鼠 脊髓损伤 鞘内置管 蛛网膜下腔 

分 类 号：R744[医药卫生—神经病学与精神病学]