长江口邻近海域沉积物中厌氧氨氧化细菌分布特征研究  

Distribution Characteristics of Anaerobic Ammonia Oxidation Bacteria in Sediments from the Adjacent Seas of Yangtze Estuary

作　　者：付璐璐[1,2,3] 甄毓[2,3,4] 贺惠[1,2,3] 张玉[2,3,4] 米铁柱[2,3,4] 

机构地区：[1]中国海洋大学海洋生命学院,青岛266003 [2]海洋环境与生态教育部重点实验室,青岛266100 [3]青岛海洋科学与技术国家实验室,海洋生态与环境科学功能实验室,青岛266071 [4]中国海洋大学环境科学与工程学院,青岛266100

出　　处：《环境科学》2016年第10期3914-3922,共9页Environmental Science

基　　金：国家自然科学基金创新研究群体项目(41521064);中国科学院海洋生态与环境科学重点实验室、青岛海洋科学与技术国家实验室海洋生态与环境科学开放基金项目(KLMEES201601)

摘　　要：海洋生态系统氮素循环对维持海洋生态收支平衡具有重要意义,由厌氧氨氧化细菌(anaerobic ammonia-oxidizing bacteria,ANAMMOX Bacteria)推动的厌氧氨氧化过程是海洋氮素移除的重要途径.本研究采用荧光定量PCR(q PCR)技术,并结合环境理化因子,分析了2011年夏季长江口邻近海域沉积物中厌氧氨氧化细菌的种群丰度分布特征.结果表明,厌氧氨氧化细菌的种群丰度在长江口邻近海域的空间分布与环境因子密切相关,总有机碳含量、盐度、铵盐浓度对长江口邻近海域沉积物中厌氧氨氧化细菌的种群丰度具有重要调控作用.厌氧氨氧化细菌的丰度分布与总有机碳含量呈显著正相关关系(P<0.01,r=0.69),同时又随亚硝酸盐浓度、溶解氧浓度的升高而降低,而盐度对厌氧氨氧化细菌丰度分布的影响较为复杂.Marine nitrogen cycle is of great significance to maintain the marine ecological balance. Anaerobic ammonia oxidation（ ANAMMOX） process driven by anaerobic ammonia-oxidizing bacteria is an important way of promoting the marine nitrogen removal.In this study,quantitative PCR（ q PCR）,combining with environmental factors analysis,were used to study the distribution of ANAMMOX bacteria in the sediments from Yangtze River Estuary and its adjacent waters in summer,2011. The results showed that the spatial abundance distribution of ANAMMOX bacteria was closely related to environmental factors. Total organic carbon,salinity,and concentration of ammonium played vital roles in regulating ANAMMOX bacteria abundances in this area. The abundance distribution of ANAMMOX bacteria was significantly positively correlated with total organic carbon content（ P 〈0. 01,r = 0. 69）,reducing along with the increasing concentration of nitrite and DO,while the influence from salinity was complicated.

关 键 词：沉积物 厌氧氨氧化细菌 amx-16SrDNA 环境因子 丰度分布 

分 类 号：X172[环境科学与工程—环境科学]