城市生活污水和生活垃圾渗滤液抗生素抗性基因污染的比较研究  

Comparative Investigation of Antibotic Resistance Genes Between Wastewater and Landfill Leachate

作　　者：黄福义[1] 李虎[1,2] 安新丽[1,2] 欧阳纬莹 苏建强[1] 

机构地区：[1]中国科学院城市环境研究所城市环境与健康重点实验室,厦门361021 [2]中国科学院大学,北京100049

出　　处：《环境科学》2016年第10期3949-3954,共6页Environmental Science

基　　金：国家重点研发计划重点专项(2016YFD0800205);国家自然科学基金项目(31270153);国家自然科学基金国际(地区)合作交流项目(21210008)

摘　　要：为研究城市生活污水(接收生活垃圾渗滤液后合并进入污水厂处理)和垃圾渗滤液中抗生素抗性的分布格局,采用高通量荧光定量PCR技术对城市生活污水和生活垃圾渗滤液中的抗生素抗性基因的多样性和丰度进行了分析.结果表明,生活垃圾渗滤液中检测出39种抗性基因,城市生活污水中检测出187种抗性基因,两者检出的抗生素抗性种类有显著差异(P<0.05);相对于垃圾渗滤液,城市生活污水抗生素抗性基因有119种显著增加(P<0.05),其中增加倍数最大的是转座子tnp A-04基因,blaVEB基因,分别达到了3 338倍和1 061倍,表明可移动元件转座子基因和β-内酰胺类抗生素抗性基因在城市生活污水中得到有效富集和传播;城市生活污水是环境抗生素抗性基因的重要存储库.The increasing output of wastewater and landfill leachate during the rapid urbanization exacerbated the human health risks induced by antibiotic resistance genes（ ARGs）. For further insight into antibiotic resistance genes（ ARGs） of wastewater（ adding leachate before treatment） and landfill leachate,high-throughput q PCR technique was used to investigate their abundance and diversities. A total of 187 unique ARGs were detected in wastewater,which was significantly higher than that in leachate（ 39 unique ARGs were detected）（ P 〈0. 05）. A total of 119 antibiotic resistance genes were significantly enriched in wastewater compared with the landfill leachate（ P 〈0. 05）,especially the tnp A-04 and blaVEB,which belongs to mobile gene elements（ MGEs） and β-Lactamase,increased by 3 338-fold and 1 061-fold,respectively. Our results showed that the profile of ARGs was significantly different between the wastewater and leachate,and the wastewater was the important reservoir of ARGs.

关 键 词：城市生活污水 生活垃圾渗滤液 抗生素 抗生素抗性基因 高通量定量PCR 

分 类 号：X171.5[环境科学与工程—环境科学]