NT-3转染骨髓间充质干细胞对脊髓缺血再灌注的机制研究  

The role of bone marrow mesenchymal stem cells transfected with neurotrophin-3 in treatment of spinal cord ischemia-reperfusion injury

作　　者：宗海斌[1] 董玉珍[2] 

机构地区：[1]新乡医学院基础医学院技能实验室,河南省新乡市453003 [2]新乡医学院第一附属医院骨外科,河南省卫辉市453100

出　　处：《实用医学杂志》2016年第19期3160-3164,共5页The Journal of Practical Medicine

基　　金：河南省医学科技攻关计划项目课题(编号:201403141)

摘　　要：目的:研究神经营养素-3(NT-3)转染骨髓间充质干细胞(BMSCs)移植对脊髓缺血再灌注损伤的作用机制。方法:将NT-3转染入BMSCs后移植于80只脊髓缺血再灌注损伤大鼠,分为4组:假手术(Sham)组、对照(NS)组、BMSCs组和NT-3+BMSCs组,观察损伤大鼠的BBB评分、神经元细胞的凋亡及丙二醛(MDA)含量和髓过氧化物酶(MPO)活性情况。结果:BMSCs+NT-3组大鼠BBB评分较BMSCs组增加,而BMSCs组较NS组增高,但Sham组明显高于BMSCs+NT-3组;BMSCs组神经元凋亡较NS组减少,BMSCs+NT-3组较BMSCs组减少,但Sham组凋亡明显低于BMSCs+NT-3组;NS组MDA及MPO活性明显高于BMSCs组,BMSCs+NT-3组两者明显低于BMSCs组,但Sham组最低。所有结果组间比较差异均有统计学意义(P<0.05)。结论:BMSCs经NT-3转染后相互促进,移植于急性脊髓缺血再灌注损伤通过减缓神经元细胞凋亡、抑制自由基的脂质过氧化反应进行修复。Objective To observe the mechanisms of bone marrow mesenchymal stem cells （BMSCs） transfected with neurotrophin-3 in treating nanoparticle repaired spinal cord ischemia-reperfusion （I/R） injury of adult rat. Methods BMSCs were transfected into NT-3 with nanoparticle carrier and transplanted into 80 adult rats on the spinal cord I/R injury model. Then the rats were divided into four groups： sham group, NS group, BMSCs and BMSCs＋NT-3. The BBB scoring system, the neurons cell apoptosis, levels of malondialdehyde （MDA） and myeloperoxidase （MPO） were observed and compared between the four groups. Results The BBB score of the BMSCs＋NT-3 group was significantly higher than those of BMSCs and NS group , but was lower obviously than the Sham group. TUNEL-apoptosis cells were significantly decreased in the BMSCs ＋ NT-3 group compared to BMSCs and NS groups, but was more significantly lower than the Sham group. The contents of MDA and MPO of the NS group were significantly higher than the BMSCs group. The contents of the BMSCs ＋ NT-3 group were lower than those of the BMSCs group, but lower significantly than the Sham group （P 〈 0.05）. Conclusion BMSCs transfected with NT-3 with nanoparticle carrier could be induced each other. When transplanted into SCI , they can repair spinal cord by alleviating neuron cells apoptosis and inhibiting the lipid peroxidation of free radicals.

关 键 词：神经营养素-3 骨髓间充质干细胞 神经元凋亡 脊髓缺血再灌注损伤 机制 NEUROTROPHIN-3 

分 类 号：R651.2[医药卫生—外科学]