贮藏温度和种子含水量对扁穗冰草种子质量的影响  

Effect of storage temperature and moisture content on the seed's quality of Agropyron cristatum

作　　者：杨晗[1] 刘鸿飞[1] 杨合龙[1] 戎郁萍[1] 

机构地区：[1]北京市草业科学重点实验室,中国农业大学动物科技学院,北京100193

出　　处：《草业科学》2016年第10期2033-2040,共8页Pratacultural Science

基　　金：国家牧草产业技术体系(CARS-35)

摘　　要：以扁穗冰草（Agropyron cristatum）种子为试验材料,研究不同温度和含水量贮藏10个月后对种子发芽率和萌发过程中生理生化变化的影响。结果表明,贮藏温度和种子含水量对发芽率、根长和芽长均具有极显著影响（P〈0.01）,而对丙二醛含量影响均不显著（P〉0.05）。扁穗冰草种子在-15℃且含水量为6%的条件下发芽率最高,为79.8%,在室温（20℃）且种子含水量为12%时,发芽率为0。扁穗冰草种子在4℃且含水量为6%的条件下胚根最长;在-15℃且含水量为12%的条件下胚芽最长。贮藏温度在20℃条件下,含水量为6%和12%的种子胚根、胚芽分别表现为最短和没有发芽。扁穗冰草种子在20℃且含水量为12%的条件下电导率最大;在4℃且含水量为4%的条件下电导率最小。相关分析表明,胚根与胚芽极显著正相关（P〈0.01）,与电导率显著正相关（P〈0.05）,其它各指标间均不显著相关（P〉0.05）。综合各项活力指标,扁穗冰草种子的适宜贮藏含水量为4%～6%,最佳贮藏温度为-15～4℃。Seed germination of Agropyron cristatum and its physiological and biochemical characteristics during germination were studied to understand the effects of the 10-month storage condition on the seed vigor.The results revealed that storage temperature and moisture content had extremely significant effects on the germination rate,root and bud lengths of A.cristatum（P〈0.01）,while MDA was not significant.The greatest seed germination rate of A.cristatum was 79.8% under the storage temperature of-15 ℃ with 6% of seed moisture content.No germination occurred at all when the seeds were stored at the temperature of 20 ℃ with 12%moisture content in the seeds.The greatest root length of A.cristatum was observed under the storage temperature of 4 ℃ with 6% of seed moisture content;The greatest bud length was observed under the storage temperature of-15℃ with 12% of seed moisture content.The shortest root and bud lengths of A.cristatum was observed under the storage temperature of 20 ℃ with 6% of seed moisture content.The greatest conductivity of A.cristatum was observed under the storage temperature of 20℃ with 12%of seed moisture content.While the least conductivity was observed under the storage temperature of 4 ℃ with 4% of seed moisture content（P〈0.01）.By analyzing the relationship between different vigor indexes of A.cristatum,we found that the relationship between root length and bud length showed extremely significant correlation（P〈0.01）.The relationship between root length and conductivity showed significant correlation（P〈0.05）,while other indexes were not significant.Taking all the vitality indexes into account,the optimum moisture content of A.cristatum was 4%～6%,the optimum storage temperature was-15～4 ℃.

关 键 词：扁穗冰草 贮藏温度 种子含水量 种子活力 

分 类 号：S543.9[农业科学—作物学]