原肌球蛋白3与雄激素受体配体结合域之间的相互作用  

Interaction of Tropomyosin Alpha-3 Chain with the Ligand-binding Domain of the Androgen Receptor

作　　者：邱阳[1] 陈晓雅[1] 孙峥嵘[2] 胡冰青 徐品一[1] 崔磊[1] 李玲[1] 

机构地区：[1]中国医科大学附属盛京医院内分泌科,沈阳110004 [2]中国医科大学附属盛京医院病毒室,沈阳110004

出　　处：《中国医科大学学报》2016年第11期973-976,共4页Journal of China Medical University

基　　金：国家自然科学基金(81170779)

摘　　要：目的为明确雄激素对动脉粥样硬化的影响及机制,在单核细胞内筛选与雄激素受体结合的蛋白。方法将雄激素受体配体结合域(AR-LBD)做为诱饵蛋白,利用酵母双杂交系统从男性单核细胞cDNA文库中筛选与AR-LBD相互作用的蛋白,进一步应用Pull-down、免疫荧光技术验证二者在体外的相互作用。结果从男性单核细胞cDNA文库中筛选出原肌球蛋白3(TPM3)与AR-LBD存在相互作用。在体外通过Pull-down实验证实了TPM3与AR-LBD的相互作用,进一步通过共聚焦显微镜检测免疫荧光确认TPM3与AR-LBD在293HEK细胞中重叠共表达。结论在男性外周血单核细胞内,TPM3与AR-LBD存在相互作用。Objective To explore the possible role and mechanisms of the androgen receptor （AR） in atherosclerosis, we screened the protein that interacted with the AR ligand-binding domain （LBD）. Methods A yeast two-hybrid system was adopted using the LBD as bait to find the new proteins, which may interact with the AR-LBD in a eDNA library of male peripheral blood monocyte. Then this interaction was further verified by Pull-down and co-localization experiment by immunofluorescence. Results We fotmd a novel protein tropomyosin alpha-3 chain which inter- acted with the LBD of the AR. The interaction of the AR-LBD with tropomyosin alpha-3 chain was confirmed by a glutathione S-transferase （GST） pull-down assay. Furthermore, co-localization experiments by fluorescent confocal microscopy revealed that the AR-LBD co-localized with tropomy- osin alpha-3 chain in 293HEK cells. Conclusion An interaction between the AR-LBD and tropomyosin alpha-3 chain in male peripheral blood monocyte may provide insights into the role and mechanisms of the signaling pathway of the androgen-AR in atherosclerosis.

关 键 词：雄激素受体 原肌球蛋白 单核细胞 酵母双杂交 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]