下调CD147表达对甲状腺癌细胞SW579运动能力的影响  

Effects of downregulation of CD147 in the mobility of thyroid cancer cell SW579 and its mechanisms

作　　者：王巍[1] 邵跃[1] 宋晓雯[1] 赵成海[1] 

机构地区：[1]中国医科大学基础医学院病理生理学教研室,沈阳市110122

出　　处：《实用医学杂志》2016年第20期3335-3338,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81502107);辽宁省自然科学基金项目(编号:2014021036)

摘　　要：目的:检测CD147 siRNA对甲状腺癌SW579细胞的作用及其机制。方法:检测CD147 siRNA处理后SW579细胞的CD147 mRNA和蛋白水平。利用CKK-8实验检测细胞增殖。同时利用transwell检测细胞运动能力变化。并利用Western-blot技术检测运动相关蛋白的变化。结果:下调CD147可以明显抑制SW579细胞增殖,并且阻碍其运动。处理后细胞的MMP-2和MMP-9蛋白水平明显下降。一种运动相关蛋白WAVE2的表达随着CD147的下调而下降。结论 :CD147下调可能通过抑制MMP-2、MMP-9和WAVE2表达导致SW579细胞运动能力下降。Objective To detect the effects and mechanisms of CD147 in thyroid cancer cell SW579.Methods RT-PCR and Western-blot were used to determine the levels of CD147 mRNA and protein in SW579 cells after treated with CD147 specific small interference RNA（siRNA）. The proliferative ratio of SW579 cells after treated with CD147 si RNA was detected by CCK-8 assay. We detected the changes of cellular mobility after treated with CD147 siRNA by transwell assay. Mobility related proteins were analyzed by western-blot.Results The proliferative ratio and the mobility of SW579 cells were inhibited significantly by down-regulation of CD147. The levels of MMP-2 and MMP-9 were lower in treated cells than untreated ones. Expression of Wave 2 and a mobility related protein were decreased in accompany with down-regulation of CD147. Conclusion Downregulation of CD147 inhibits mobility of SW579 by suppressing MMP-2, MMP-9 and WAVE 2.

关 键 词：SW579细胞 CD147 SIRNA 运动能力 WAVE 2 

分 类 号：R736.1[医药卫生—肿瘤]