血管紧张素Ⅱ对巨噬细胞移动抑制因子表达的影响  

Effect of angiotensin Ⅱ on the expression of MIF in macrophages

作　　者：王艳宁[1] 王旭光[1] 张珉[1] 韩莹[1] 吴凡[1] 

机构地区：[1]沈阳医学院基础医学院病理教研室,110034

出　　处：《实用医学杂志》2016年第20期3372-3375,共4页The Journal of Practical Medicine

基　　金：辽宁省科技厅课题(编号:2013225086)

摘　　要：目的:探讨血管紧张素Ⅱ(AngⅡ)是否能通过核因子-κB(NF-κB)途径调节巨噬细胞移动抑制因子(MIF)的表达。方法:佛波酯诱导THP-1细胞48 h使其转化为巨噬细胞。第一组:对照组:AngⅡ1×10^(-8)mol/L组;AngⅡ1×10^(-7)mol/L组;AngⅡ1×10^(-6)mol/L组;AngⅡ1×10^(-5)mol/L组。第二组:对照组;AngⅡ1×10^(-6)mol/L组:AngⅡ1×10^(-6)mol/L+PDTC组:PDTC组。Western blot检测第一组细胞核内NF-κB的表达。Real time RT-PCR检测第二组巨噬细胞MIF mRNA的表达。ELISA检测第二组巨噬细胞上清液中MIF的含量。结果:Western Blot:随着AngⅡ浓度的增高,NF-κBp65的表达逐渐增强。Real time RT-PCR:AngⅡ组MIF mRNA表达量明显高于对照组:PDTC作用后,由AngⅡ诱导的MIF mRNA表达明显降低。ELISA:AngⅡ组MIF含量明显高于对照组;PDTC作用后,由AngⅡ诱导增加的MIF含量明显降低。结论:AngⅡ能通过NF-κB信号通路促进巨噬细胞MIF mRNA的表达和MIF的分泌。Objective To investigate whether angiotensin Ⅱ（AngⅡ） can regulate the expression of MIF in macrophages via the NF-κB pathway. Methods Western Blot, real time RT-PCR and ELISA were used in the present study. Results Western blot result showed that the expression of NF-κBp65 gradually increased with the increase of the concentration of Ang Ⅱ. Results of real time RT-PCR and ELISA revealed that MIF m RNA expression and the content of MIF were significantly higher in Ang Ⅱ group than those in the control group. PDTC could reverse the effect of Ang Ⅱ on MIF m RNA expression and MIF secretion in macrophages.Conclusion Ang Ⅱ can promote MIF m RNA expression and MIF secretion in macrophages via the NF-κB signaling pathway.

关 键 词：动脉粥样硬化 血管紧张素Ⅱ 巨噬细胞移动抑制因子 NF-ΚB 

分 类 号：R543.5[医药卫生—心血管疾病]